Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   JFL55_RS07245 Genome accession   NZ_CP066564
Coordinates   1571283..1571822 (-) Length   179 a.a.
NCBI ID   WP_012341221.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-69     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1566283..1576822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFL55_RS07225 (JFL55_07225) - 1567714..1567860 (-) 147 WP_157665520.1 hypothetical protein -
  JFL55_RS07230 (JFL55_07230) - 1567860..1568075 (-) 216 WP_075294140.1 transposase -
  JFL55_RS07235 (JFL55_07235) - 1568184..1568441 (+) 258 WP_075294154.1 helix-turn-helix domain-containing protein -
  JFL55_RS07240 (JFL55_07240) nrdA 1568930..1571200 (+) 2271 WP_012341222.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  JFL55_RS07245 (JFL55_07245) pilF/pilF2 1571283..1571822 (-) 540 WP_012341221.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JFL55_RS07250 (JFL55_07250) - 1571853..1572971 (-) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  JFL55_RS07255 (JFL55_07255) dnaX 1573224..1575227 (-) 2004 WP_012341219.1 DNA polymerase III subunit gamma/tau -
  JFL55_RS07260 (JFL55_07260) apt 1575258..1575797 (-) 540 WP_198974110.1 adenine phosphoribosyltransferase -
  JFL55_RS07265 (JFL55_07265) lpxM 1575868..1576821 (-) 954 WP_012341217.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20670.59 Da        Isoelectric Point: 9.3139

>NTDB_id=520954 JFL55_RS07245 WP_012341221.1 1571283..1571822(-) (pilF/pilF2) [Histophilus somni strain ASc-MMNZ-VFA-69]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=520954 JFL55_RS07245 WP_012341221.1 1571283..1571822(-) (pilF/pilF2) [Histophilus somni strain ASc-MMNZ-VFA-69]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(109-130)

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436