NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	JEQ22_RS12635	Genome accession	NZ_CP066501
Coordinates	2568815..2569429 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain ID-A02
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2563815..2574429

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JEQ22_RS12605 (JEQ22_12530)	rpsT	2564118..2564384 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JEQ22_RS12610 (JEQ22_12535)	holA	2564347..2565442 (-)	1096	Protein_2436	DNA polymerase III subunit delta	-
JEQ22_RS12615 (JEQ22_12540)	-	2565482..2565633 (-)	152	Protein_2437	hypothetical protein	-
JEQ22_RS12620 (JEQ22_12545)	-	2565674..2565808 (+)	135	WP_003152870.1	YqzM family protein	-
JEQ22_RS12625 (JEQ22_12550)	comEC	2565827..2568178 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JEQ22_RS12630 (JEQ22_12555)	-	2568179..2568748 (-)	570	WP_003152868.1	ComE operon protein 2	-
JEQ22_RS12635 (JEQ22_12560)	comEA	2568815..2569429 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
JEQ22_RS12640 (JEQ22_12565)	comER	2569488..2570309 (+)	822	WP_014305449.1	late competence protein ComER	-
JEQ22_RS12645 (JEQ22_12570)	-	2570378..2571115 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
JEQ22_RS12650 (JEQ22_12575)	rsfS	2571112..2571468 (-)	357	WP_003152864.1	ribosome silencing factor	-
JEQ22_RS12655 (JEQ22_12580)	yqeK	2571465..2572046 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JEQ22_RS12660 (JEQ22_12585)	-	2572036..2572605 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
JEQ22_RS12665 (JEQ22_12590)	yhbY	2572616..2572906 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JEQ22_RS12670 (JEQ22_12595)	aroE	2572900..2573742 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=520541 JEQ22_RS12635 WP_013352942.1 2568815..2569429(-) (comEA) [Bacillus velezensis strain ID-A02]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=520541 JEQ22_RS12635 WP_013352942.1 2568815..2569429(-) (comEA) [Bacillus velezensis strain ID-A02]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0520541