Detailed information NTG0519756

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JEQ24_RS14005
• Genome accession: NZ_CP066379
• Coordinates: 2783845..2784459 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain ID-A04
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2778845..2789459

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JEQ24_RS13975 (JEQ24_13825)	rpsT	2779148..2779414 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JEQ24_RS13980 (JEQ24_13830)	holA	2779431..2780472 (-)	1042	Protein_2708	DNA polymerase III subunit delta	-
JEQ24_RS13985 (JEQ24_13835)	-	2780512..2780663 (-)	152	Protein_2709	hypothetical protein	-
JEQ24_RS13990 (JEQ24_13840)	-	2780704..2780838 (+)	135	WP_003152870.1	YqzM family protein	-
JEQ24_RS13995 (JEQ24_13845)	comEC	2780857..2783208 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JEQ24_RS14000 (JEQ24_13850)	-	2783209..2783778 (-)	570	WP_003152868.1	ComE operon protein 2	-
JEQ24_RS14005 (JEQ24_13855)	comEA	2783845..2784459 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
JEQ24_RS14010 (JEQ24_13860)	comER	2784518..2785339 (+)	822	WP_014418420.1	late competence protein ComER	-
JEQ24_RS14015 (JEQ24_13865)	-	2785408..2786145 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
JEQ24_RS14020 (JEQ24_13870)	rsfS	2786142..2786498 (-)	357	WP_007408260.1	ribosome silencing factor	-
JEQ24_RS14025 (JEQ24_13875)	yqeK	2786516..2787076 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JEQ24_RS14030 (JEQ24_13880)	-	2787066..2787635 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
JEQ24_RS14035 (JEQ24_13885)	yhbY	2787646..2787936 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JEQ24_RS14040 (JEQ24_13890)	aroE	2787930..2788772 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=519756 JEQ24_RS14005 WP_014418419.1 2783845..2784459(-) (comEA) [Bacillus velezensis strain ID-A04]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=519756 JEQ24_RS14005 WP_014418419.1 2783845..2784459(-) (comEA) [Bacillus velezensis strain ID-A04]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363