Detailed information NTG0519680

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JEQ23_RS14300
• Genome accession: NZ_CP066378
• Coordinates: 2825445..2826059 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain ID-A03
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2820445..2831059

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JEQ23_RS14270 (JEQ23_14115)	rpsT	2820748..2821014 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JEQ23_RS14275 (JEQ23_14120)	holA	2821031..2822072 (-)	1042	Protein_2766	DNA polymerase III subunit delta	-
JEQ23_RS14280 (JEQ23_14125)	-	2822112..2822263 (-)	152	Protein_2767	hypothetical protein	-
JEQ23_RS14285 (JEQ23_14130)	-	2822304..2822438 (+)	135	WP_003152870.1	YqzM family protein	-
JEQ23_RS14290 (JEQ23_14135)	comEC	2822457..2824808 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JEQ23_RS14295 (JEQ23_14140)	-	2824809..2825378 (-)	570	WP_003152868.1	ComE operon protein 2	-
JEQ23_RS14300 (JEQ23_14145)	comEA	2825445..2826059 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
JEQ23_RS14305 (JEQ23_14150)	comER	2826118..2826939 (+)	822	WP_014418420.1	late competence protein ComER	-
JEQ23_RS14310 (JEQ23_14155)	-	2827008..2827745 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
JEQ23_RS14315 (JEQ23_14160)	rsfS	2827742..2828098 (-)	357	WP_007408260.1	ribosome silencing factor	-
JEQ23_RS14320 (JEQ23_14165)	yqeK	2828116..2828676 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JEQ23_RS14325 (JEQ23_14170)	-	2828666..2829235 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
JEQ23_RS14330 (JEQ23_14175)	yhbY	2829246..2829536 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JEQ23_RS14335 (JEQ23_14180)	aroE	2829530..2830372 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=519680 JEQ23_RS14300 WP_014418419.1 2825445..2826059(-) (comEA) [Bacillus velezensis strain ID-A03]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=519680 JEQ23_RS14300 WP_014418419.1 2825445..2826059(-) (comEA) [Bacillus velezensis strain ID-A03]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363