Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   I9H08_RS03310 Genome accession   NZ_CP066263
Coordinates   761841..763433 (-) Length   530 a.a.
NCBI ID   WP_003403505.1    Uniprot ID   A0A0L8IK44
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 756841..768433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS03295 (I9H08_03275) - 758857..759258 (+) 402 WP_011266587.1 type IV pilin protein -
  I9H08_RS03300 (I9H08_03280) thiO 759285..760388 (+) 1104 WP_103360183.1 glycine oxidase ThiO -
  I9H08_RS03305 (I9H08_03285) pilR 760501..761838 (-) 1338 WP_011266589.1 sigma-54 dependent transcriptional regulator Regulator
  I9H08_RS03310 (I9H08_03290) pilS 761841..763433 (-) 1593 WP_003403505.1 ATP-binding protein Regulator
  I9H08_RS03315 (I9H08_03295) - 763423..763662 (-) 240 WP_103360184.1 PP0621 family protein -
  I9H08_RS03320 (I9H08_03300) - 763783..764805 (-) 1023 WP_003371138.1 outer membrane protein assembly factor BamD -
  I9H08_RS03325 (I9H08_03305) rluD 764955..765917 (+) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  I9H08_RS03330 (I9H08_03310) pgeF 765914..766642 (+) 729 WP_103360185.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 57986.92 Da        Isoelectric Point: 7.0011

>NTDB_id=518966 I9H08_RS03310 WP_003403505.1 761841..763433(-) (pilS) [Pseudomonas syringae pv. syringae strain Pss9644]
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQGALHLLGQEQLVGQIID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QKIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNDQKSDLPTLEVLDDGPGVSEQHLSKMFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=518966 I9H08_RS03310 WP_003403505.1 761841..763433(-) (pilS) [Pseudomonas syringae pv. syringae strain Pss9644]
GTGACCGCTGACTCACCGTCCCTTGTCAGCCCGCAGGCACAGCGGATTCTACGGCTGTACCACCTCTACCGACTGACCAT
CGGCGTTGTACTGGTTCTGTTGATTTCCAGCAGCCTGGAAACCGAGCTGCTGCAACTGGCCAACCCGAACCTGTTCCGCT
CGGGCTGCTGGTTGTATCTGGTGTTCAACATTCTGGTCGTCGTGCTGCTGGAACGCCCCAGCCGTCAGGCACAGTTTTTC
GGCCTGGCGATGACCGACGCGATCATGCTCTCCGCGCTGTTCTACGCAGCCGGCGGGCCTTCCAGCGGTATCGGCAACCT
GTTGATTGCCTCGGTGGCGATCAGCAACGTGCTGCTACGCGGCCGTATCGGTCTGCTGATCGCTGCGGTTTCGGCCATCG
GCATCATCTACCTGACGTTCTACATCAGCTTCGGCACCCCCTCGGTTTCCAACGACTACGTGCAGGCTGGCTCGCTGGGC
GCCTTGTGCTTCGCCGCCGCCCTGCTGGTGCAGGCCCTGACAGCGCGCATGCAGGTCAGCGAGGTGCTGGCCGAACAGCG
CGCGGCGGATGTCGACAGCCTTGAAGCACTCAACGCGCTGATCCTGCAACGCATGCGTACCGGCATTCTGGTGCTGGATG
CCAACCATCAGGTGCTGCTGGCCAATCAGGGGGCCTTGCACCTGCTGGGCCAGGAACAACTGGTCGGCCAGATCATCGAC
ACCTGCTCGCCACAACTGGTCGAACGCCTGCGCCAGTGGCTCGTCAACCCGATCATGGTGCCGCGCAGCATCACCACCGC
CCCCGTCGGGCTGGTACTGCAACCGAGTTTTGCCACACTCAACCGTGGCGATCAGCGTCAGACGCTGGTTTTTCTCGAAG
ACCTGTCGCAGATCGCCCATCAGGCGCAGCAGCTCAAGCTTGCCGCACTGGGGCGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCCGTCAGCCATGCGGCGCAGTTGCTTAATGAATCGGAAGAGCTCAGTGCGGCGGATCGACG
CCTGGGGCAGATCATTCATGAGCAGTCGGGACGCATTGATAGGGTCATCGAAAATGTCCTGCAGTTGTCGCGCAGACGCG
AGGCGCAACCCGAACTGCTCGATCTGAAAACCTGGCTGGAACACTTTGTCGCTGAATTTCGCAGTTCCGCTGCGCCCAAT
CAGAAGATTCATGTCGACATCAGCCCTGGCACGCTGACAACCCGCATGGATTCCGGACAACTGACGCAAATCATGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGAATCATCCGTCAGCGCAGGTCTGGCTGAAACTACACAATGACCAGA
AGAGCGACCTGCCCACACTGGAAGTGCTGGACGACGGGCCGGGGGTCAGCGAGCAACACCTGAGCAAAATGTTCGAACCG
TTTTTCACCACAGAAAGCAAAGGCACCGGCCTTGGTCTATATCTGTCACGTGAACTGTGTGAAAGCAATCAGGCGCATTT
GGAATACGCGCCCCGCCCCACGGGTGGCAGTTGCCTGCGCATTACCTTCGCCCACCCGTTCAAGACGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

PAS domain (PAS_8)

(202-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L8IK44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.017

100

0.611