Detailed information NTG0518488

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: JD965_RS05890
• Genome accession: NZ_CP066219
• Coordinates: 1121697..1125401 (+)
• Length: 1234 a.a.
• NCBI ID: WP_198698950.1
• Uniprot ID: -
• Organism: Bacillus siamensis strain B28
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1116697..1130401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JD965_RS05875 (JD965_05875)	-	1116777..1117286 (-)	510	WP_198698655.1	ferritin-like domain-containing protein	-
JD965_RS05880 (JD965_05880)	-	1117355..1118065 (-)	711	WP_064777801.1	DUF421 domain-containing protein	-
JD965_RS05885 (JD965_05885)	addB	1118210..1121710 (+)	3501	WP_198698656.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
JD965_RS05890 (JD965_05890)	addA	1121697..1125401 (+)	3705	WP_198698950.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
JD965_RS05895 (JD965_05895)	sbcD	1125467..1126639 (+)	1173	WP_047476740.1	exonuclease subunit SbcD	-
JD965_RS05900 (JD965_05900)	sbcC	1126636..1130028 (+)	3393	WP_198698657.1	exonuclease subunit SbcC	-
JD965_RS05905 (JD965_05905)	-	1130045..1130344 (+)	300	WP_047476743.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141303.72 Da
• Isoelectric Point: 5.2245

>NTDB_id=518488 JD965_RS05890 WP_198698950.1 1121697..1125401(+) (addA) [Bacillus siamensis strain B28]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALIGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFGALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAADEDGRRMPSEAAVYYQEQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNRNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNEAAETELLLIDSADDADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDELYQKLRTFYDNLQKWRSFSTNHSVAELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNREKQLAKWQAQADRADWLLSEFDRYQAASYLDFIGPALIRHRDMEAHRPPGLSAREDIAHDPSRFHIRMLQQSE
LLEDNPKERAEEKSKRLKAIQQGEPIPDSFSFDEQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPSAEEAERTVRTLHEKELLTEEQRDAIDIDDIIQFFETEIGK
NLLGARRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAIEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3705 bp

>NTDB_id=518488 JD965_RS05890 WP_198698950.1 1121697..1125401(+) (addA) [Bacillus siamensis strain B28]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACCGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTCCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGCTTTCGAATGGCTG
ATCAGACGGAAGGCGAATTGCTCGGAGACGAAGTCCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATTTGGATCTTCAAGATCTCGTCAAGCGGGTGTACGAGTA
TTCACGGTCTCATCCTGATCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTGCCATTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCATCGGGGCAAAGCAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGCGCTGATAATTTTCTGGATGATCTTCAGCAGATAGAAGAGTT
GATCAGCTGCCGGCATGACTTTGGGGCGCTGTATGAACGGGTTCCCGCGGTCACATTTAAGCGGGCTAAGGCGGTAAAAG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCCACTGATTTGCGAAACGGCGCGAAAAAACTGATCGAAAAGTTAAAG
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAACAGACATGAAGCCTGTCATTGAAACGCTCGTTCA
GCTCGTGATATCGTATGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCCATTTTAACTGCGGCTGATGAAGACGGCCGGCGGATGCCGAGTGAAGCGGCCGTTTATTATCAAGAGCAG
TTCCATGAGGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCTATTCTGCAGCTTGTAAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCCGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGACAGAAAATTGACCTGAACCGAAACTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGTGCGTCGTATCCGCCGAATGAGGCGGCAGAAACGGAACTGCTGCTGATTGACAGTGCTG
ATGATGCAGACAGCTCCGAGGATGCCGAAGATTTTGAAACCGTGCATTGGGAAGCAAAAGCGATAGCCGGGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTCTATGACGGCAAAACCAAAACACACCGGAATATCCAGTATAGGGATATCGTGAT
TTTGCTGCGTTCCATGCCTTGGGCGCCGCAATTGATGGAAGAACTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCAGGTTATTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCGGTCTTAAAGGTGATTGACAACCCGTATCAGGATATT
CCGCTTGCTTCCGTACTGCGCTCGCCCATTGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTGGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGGTGACAGACATGACGAGCTGTATCAAAAGCTGAGAACTT
TTTATGACAATCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCGGTAGCTGAGCTGATTTGGGAAGTATACCGCGAT
ACAGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATTCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTGTTATATGTAGCGCTGACAAGAGCGAAGGAAAAGCTGTTTCTC
ATCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCGGACAGGGCCGATTGGCTTCTCTCTGA
ATTCGACCGCTATCAGGCGGCATCTTATTTGGATTTTATCGGGCCGGCGCTTATCCGCCACCGTGACATGGAGGCACACA
GGCCGCCGGGTCTTTCGGCTCGGGAGGATATCGCGCACGACCCGTCCCGTTTTCATATCCGGATGCTGCAGCAAAGTGAA
CTGCTGGAAGATAACCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGAACAGGCGCGCCGTCTCCTTGAATGGGAATACCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCCCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGTTTTATGATGAAAAAGGGGCTGACGGCCGCGGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGAGTGCTGAAGAAGCGGAGCGGACGGTTCGGACGCTTCACGAAA
AAGAGCTGCTGACAGAAGAACAGCGGGACGCTATTGATATCGACGACATTATACAGTTTTTCGAAACTGAAATCGGGAAA
AACCTTCTCGGGGCGCGGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAAGATGC
CGAAACGGCTGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGACTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGATTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.658	100	0.827