Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   JD965_RS05885 Genome accession   NZ_CP066219
Coordinates   1118210..1121710 (+) Length   1166 a.a.
NCBI ID   WP_198698656.1    Uniprot ID   -
Organism   Bacillus siamensis strain B28     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1113210..1126710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JD965_RS05860 (JD965_05860) - 1113408..1114565 (-) 1158 WP_198698652.1 AbrB family transcriptional regulator -
  JD965_RS05865 (JD965_05865) - 1114732..1115928 (-) 1197 WP_198698653.1 MFS transporter -
  JD965_RS05870 (JD965_05870) - 1115944..1116567 (-) 624 WP_198698654.1 TetR/AcrR family transcriptional regulator -
  JD965_RS05875 (JD965_05875) - 1116777..1117286 (-) 510 WP_198698655.1 ferritin-like domain-containing protein -
  JD965_RS05880 (JD965_05880) - 1117355..1118065 (-) 711 WP_064777801.1 DUF421 domain-containing protein -
  JD965_RS05885 (JD965_05885) addB 1118210..1121710 (+) 3501 WP_198698656.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JD965_RS05890 (JD965_05890) addA 1121697..1125401 (+) 3705 WP_198698950.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JD965_RS05895 (JD965_05895) sbcD 1125467..1126639 (+) 1173 WP_047476740.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133450.05 Da        Isoelectric Point: 5.4513

>NTDB_id=518487 JD965_RS05885 WP_198698656.1 1118210..1121710(+) (addB) [Bacillus siamensis strain B28]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADLVKGARVYVDGFYQFTPQELRVLEQLIIHAEHVTFSLTADSP
SGEQAPDELNLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACLESQYDVRPAAAYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDGGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPVQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETREW
MAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHSQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLRAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGNGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=518487 JD965_RS05885 WP_198698656.1 1118210..1121710(+) (addB) [Bacillus siamensis strain B28]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGA
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTGTTGAGGAAGCTGATTGAAGAGCATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGTTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCCGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATCTGCATTCAGAGGATTATTT
GACGCTGCTCGCCCAGCAGCTTCCGTTAGCGGATCTTGTAAAAGGCGCCAGAGTTTACGTCGACGGCTTCTATCAATTTA
CACCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATTCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGGAGAACAGGCACCGGATGAGCTCAATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAACGGCATCAGCATGTGCCGGAACTTGCCTGTTTAG
AATCGCAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGGCAGGAAGCTTTTACCGTTATACAGGCACAAAACAGA
AGAGCCGAGCTTGAGGGTATTGCCCGGGAAATCCAATCACTTGTCCGGGACGGAGGATACCGGTACCGGGATATGGCGAT
TCTCATCCGCCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATAGAATTCATCCGTTCAAGTTTGGATGTCGTAAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGTGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTATTGTATCGCCTACGGAATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAAGAAATAGAGATGGAGCAAATGCTGAATGAGACGCGAGAATGG
ATGGCCGCGCCCCTCGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGACACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCAGGCCGAAGAGGCCGGAAACATGATTGAAG
CGCAGCAGCACAGCCAGGCATGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCACTTCATTTTTCTTTAATTCCCCCTGCGCTTGACCA
AGTGTTTGTAGGGAATATGGACCTGTCAAGAATGTACGGCACGTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCCGATGATGACCGGGAGTGGCTGAGAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGCCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATTGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ACCATCAGGAAAAACTGTCGGCTGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTATGAAATCAGTGATGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCGGCGCGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGAGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTCAAGCTGATTTCAGACCGGCTTCGTGAACAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAAATTCTGCTCAGTTCAAAT
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGAGTCTCCGGCATATTGAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTTGGCGGAAACGGTCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGTCTGCTGCTTAGAATCGTG
GATTACAAATCAAGCGACAAGGGGCTTGATCTGGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACGTACCTTGA
CTTATCGATTACACATTCAGCGGATTGGCTCGGCATGAAAGCGACACCCGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGTCTT
CTCCTCGGTGACCGGGAGGCAATCAGCCTGATGGACACGACTTTGGAGGAAGGACGTTCAAATATCATTAATGCAGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGATTCAGCCGCAGTCAGCGAACGGGATTTTCATCTCCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCATTTAAATCGGTGTGCCAGTTTGATGAGTCTCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(304-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.276

100

0.833