Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   JC776_RS03220 Genome accession   NZ_CP066194
Coordinates   628338..632063 (-) Length   1241 a.a.
NCBI ID   WP_048723054.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain E8.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 623338..637063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC776_RS03175 (JC776_03175) - 623506..624696 (+) 1191 WP_048722287.1 ornithine--oxo-acid transaminase -
  JC776_RS03180 (JC776_03180) - 624805..625704 (-) 900 WP_179196689.1 fumarylacetoacetate hydrolase family protein -
  JC776_RS03185 (JC776_03185) - 625951..626130 (+) 180 WP_011983956.1 aspartyl-phosphate phosphatase Spo0E family protein -
  JC776_RS03190 (JC776_03190) - 626257..626478 (+) 222 WP_011983955.1 spore germination protein -
  JC776_RS03195 (JC776_03195) - 626496..626702 (+) 207 WP_011983954.1 spore germination protein GerPB -
  JC776_RS03200 (JC776_03200) gerPC 626763..627383 (+) 621 WP_011983953.1 spore germination protein GerPC -
  JC776_RS03205 (JC776_03205) - 627388..627582 (+) 195 WP_011983952.1 hypothetical protein -
  JC776_RS03210 (JC776_03210) - 627597..627965 (+) 369 WP_011983951.1 spore germination protein GerPE -
  JC776_RS03215 (JC776_03215) - 628030..628245 (+) 216 WP_011983950.1 spore germination protein -
  JC776_RS03220 (JC776_03220) addA 628338..632063 (-) 3726 WP_048723054.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JC776_RS03225 (JC776_03225) addB 632063..635575 (-) 3513 WP_048722281.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JC776_RS03230 (JC776_03230) lepB 635699..636265 (-) 567 WP_011983947.1 signal peptidase I -
  JC776_RS03235 (JC776_03235) - 636326..636919 (-) 594 WP_011983946.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 143828.41 Da        Isoelectric Point: 5.0777

>NTDB_id=518380 JC776_RS03220 WP_048723054.1 628338..632063(-) (addA) [Bacillus cytotoxicus strain E8.1]
MVENWPAKPEGSQWTDDQWKAVVAHGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIEEPSSRHIRKQLSLLNKASISTIHSFCLQVIRSYYYMLDIDPRFRIANQTENELLKEEVLDDILEEEYGME
ENQLFFELVDRYTSDRNDDDLQRMILALHTAAGAHPNPEKWLDRLVEAYNVEGKTIEDLMYASYLLEDVKFQLETATEHI
RKAMELAMLPDGPAPRMETLQADLVLLETLSHAARKSWTSVYEAMQHVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQEMHPVLGKLVQLVKEFSNRFQAIKRDKGMVDFTDLEHFCLQILSEQGEDGELRPSPVALQ
YRNRFAEVLVDEYQDTNFVQESIIKLVTKDSEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLEGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIEYDADAELKLGASYPEGEDVAAELLCIHQSEEEVLDGEEGEEVEKAQLEARLI
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVNIMMNVFRVIDN
PVQDIPLAAVLRSPIVGLNDEELAMLRAHAKKGSFYEVMRSFLRGAPLEGEKELHEKLKWFYHLLQGWREFARQQSLSDL
IWKVYRETGYYDFVGGLPGGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMQRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKRELIAEEMRVLYVALTR
AKEKLILIGTVKDKEKEMEKWLDTREHTEWLLPDYVRASASCYLDWIAPSLYRHRDSEILLELGQGTIPNEIYEYDTSWK
VEFVDGKTLLAPEPAQEEKQELLEALREKKAVPLESERKDEVYNRLTWKYEYEDATLQRAKQSVTEIKRNYQSEDGSDTA
FIQKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKQSITIESIQEQIAKMVNKEILTFEQAEEISVERIVAFFES
HLGKRVLEAKSVEREVPFTMMLSAKEAYQNWQGKSEETILVQGVIDCMIEEDDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKTLQHPVKEKCLYFFDGNHVITIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=518380 JC776_RS03220 WP_048723054.1 628338..632063(-) (addA) [Bacillus cytotoxicus strain E8.1]
ATGGTAGAAAATTGGCCAGCAAAACCAGAAGGGAGTCAATGGACAGATGATCAATGGAAAGCTGTTGTGGCACATGGACG
TGATATTTTAGTTGCAGCAGCAGCAGGTTCCGGGAAAACAGCAGTACTCGTTGAACGTATCATTAAAAAGATTATTAATG
AGGAGAATCCGGTAGATGTCGATCGCTTACTCGTTGTAACATTTACGAATGCAGCCGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGAGGAACCGAGTTCACGGCATATACGGAAGCAGCTGAGCTTATTAAATAAGGC
TTCCATCTCAACAATTCATTCTTTTTGTTTACAAGTCATTCGTTCATATTATTACATGCTTGATATCGATCCTCGTTTTC
GCATCGCGAATCAAACGGAAAATGAGTTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATGGAA
GAGAATCAACTATTCTTTGAACTTGTCGATCGCTATACGAGCGACCGTAATGATGATGATTTGCAGCGAATGATTTTGGC
GCTGCATACAGCAGCAGGAGCGCATCCAAATCCAGAAAAGTGGCTTGATAGATTAGTAGAAGCGTATAATGTGGAAGGAA
AAACAATTGAAGATTTAATGTATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAACGGAGCACATT
CGAAAAGCAATGGAATTAGCGATGCTTCCCGATGGTCCTGCTCCTCGCATGGAGACATTACAAGCGGATCTCGTTTTACT
AGAAACGCTATCTCATGCTGCGCGCAAGTCGTGGACAAGTGTATATGAAGCAATGCAGCATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGCGATTATAACGAAGATATTGTGAAACAAGTCGATTCTCTTCGCAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAACTGTTTAGTCGTAAACCAGAAAGCTTTTTACGTGATTTTCAAGAGATGCACCCTGTATTAGG
AAAACTCGTGCAGCTTGTTAAAGAGTTTTCGAACCGATTTCAAGCGATAAAACGTGATAAAGGAATGGTTGATTTTACAG
ATTTAGAGCATTTTTGTTTGCAAATTTTAAGCGAGCAAGGTGAAGATGGTGAACTGCGCCCATCGCCAGTTGCACTTCAA
TACCGCAATAGATTTGCGGAAGTTCTCGTTGATGAATATCAAGATACAAACTTCGTTCAAGAATCCATTATTAAACTCGT
AACGAAAGACTCTGAGCAAGAAGGTAATTTGTTTATGGTCGGTGACGTAAAACAGTCTATTTATCGCTTTCGATTAGCAG
AACCCGGCTTGTTTTTAGGAAAATATAAACGTTTTACACAAGAAGGATTAGAAGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGCAGTCGTCATGAAGTATTAGCTGGTACAAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGTGAAAT
TGAGTATGATGCTGACGCTGAATTAAAGTTAGGTGCAAGCTATCCAGAAGGAGAAGATGTAGCGGCAGAGTTATTATGCA
TTCACCAATCTGAAGAAGAAGTGCTAGATGGAGAAGAGGGTGAAGAAGTAGAAAAAGCGCAGCTGGAAGCACGTCTCATT
GCGCAGCGCATAAAAGCGATGGTTGACTCAGGATATACAGTATATGACCGGAAAACGAATGAAATGCGTCAGGTCCAATA
TCGCGATTTTGTTATTTTACTGCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAATTAAAGCTGCAAGGCATTCCAG
TATACGCAGAACTTGCAACGGGCTATTTTGAAGCGACAGAAGTAAATATTATGATGAATGTTTTCCGCGTTATTGATAAT
CCGGTGCAAGATATTCCGCTAGCAGCTGTACTTCGTTCGCCAATTGTTGGGCTAAATGATGAGGAACTTGCGATGCTTCG
TGCGCATGCAAAGAAAGGCTCTTTTTATGAGGTGATGCGCTCGTTCTTACGAGGAGCACCGCTTGAAGGAGAGAAGGAAC
TGCATGAAAAGCTAAAGTGGTTTTATCACTTACTGCAAGGATGGCGTGAGTTTGCGCGTCAGCAATCTCTTTCTGACTTA
ATTTGGAAAGTGTATCGTGAAACTGGGTATTATGACTTTGTCGGCGGATTACCTGGCGGAAAGCAACGTCAGGCAAACTT
GCGTGTTTTATACGACCGCGCAAGGCAATATGAGGCGACATCATTTAGAGGGTTATTCCGCTTTTTACGCTTTATTGAGC
GCATTTTAGAACGCGGTGATGATATGGGAACTGCAAGAGCTCTTGGAGAGCAAGAAGATGTTGTGCGCATTATGACCATT
CATAAAAGTAAAGGACTAGAGTTCCCAGTTGTGTTTGTGGCAGGGCTCGGCCGTCGTTTTAATACGCAAGATTTAATGCA
GCGTTTCTTATTGCATAAAGACTTCGGATTTGGTTCACAATTTATTGACCCGCGCAAGCGAATTAAATATACGACATTAT
CGCAATTAGCGATTAAACGAAAAATGAAACGCGAGTTAATTGCAGAAGAAATGCGTGTATTATATGTTGCGTTAACGCGT
GCAAAAGAAAAATTAATTTTAATTGGAACGGTGAAAGATAAGGAAAAAGAAATGGAAAAATGGCTTGATACACGGGAGCA
TACAGAATGGTTACTACCAGATTATGTACGCGCTAGTGCGTCATGTTATTTAGATTGGATTGCTCCTTCCTTATACCGAC
ATCGAGATAGTGAAATACTCCTTGAATTAGGACAAGGTACGATTCCAAATGAAATTTATGAATATGATACAAGCTGGAAA
GTAGAATTCGTTGATGGCAAAACGTTACTCGCACCAGAGCCAGCTCAAGAAGAAAAGCAAGAGTTGTTAGAAGCGCTGCG
TGAGAAAAAGGCTGTCCCGTTAGAGAGTGAACGAAAAGATGAAGTATATAATCGATTAACATGGAAATACGAATACGAGG
ATGCAACGCTTCAACGTGCGAAGCAATCTGTTACAGAGATTAAAAGAAATTATCAATCGGAAGATGGAAGTGATACGGCC
TTTATTCAAAAACTACGTGCGCCAATTCGAACACGTCCGCGTTTTATGGAGAAAAAAGGCTTAACATATGCAGAGCGTGG
AACAGCTGTTCATGCCGTTATGCAGCATGTTGATTTGAAGCAATCTATTACAATTGAATCTATTCAAGAGCAAATCGCAA
AAATGGTGAATAAAGAGATCTTAACCTTTGAACAAGCAGAAGAAATCAGTGTCGAAAGAATAGTGGCATTTTTTGAAAGT
CATCTTGGAAAACGAGTGTTAGAAGCGAAAAGTGTTGAGCGAGAAGTACCGTTTACGATGATGCTTTCAGCAAAAGAAGC
GTATCAAAATTGGCAAGGTAAGAGCGAAGAGACGATACTCGTACAAGGGGTTATCGACTGTATGATCGAAGAAGATGATG
GCATTACGTTAATTGACTTTAAAACAGATACAATTGAAGGGAAATTTCCAGGTGGATTCGATCAGGCGAAACCGATTTTA
GAAGAGCGCTATAAAGTACAGCTTTCCTTATATGCTAAAGCACTAGAAAAAACGTTACAACACCCTGTAAAAGAAAAGTG
TTTATACTTTTTTGATGGAAATCATGTGATTACGATTGAGGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1058-1231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.408

100

0.537