Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   JC776_RS03225 Genome accession   NZ_CP066194
Coordinates   632063..635575 (-) Length   1170 a.a.
NCBI ID   WP_048722281.1    Uniprot ID   A0AAX2CDJ0
Organism   Bacillus cytotoxicus strain E8.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 627063..640575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC776_RS03205 (JC776_03205) - 627388..627582 (+) 195 WP_011983952.1 hypothetical protein -
  JC776_RS03210 (JC776_03210) - 627597..627965 (+) 369 WP_011983951.1 spore germination protein GerPE -
  JC776_RS03215 (JC776_03215) - 628030..628245 (+) 216 WP_011983950.1 spore germination protein -
  JC776_RS03220 (JC776_03220) addA 628338..632063 (-) 3726 WP_048723054.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JC776_RS03225 (JC776_03225) addB 632063..635575 (-) 3513 WP_048722281.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JC776_RS03230 (JC776_03230) lepB 635699..636265 (-) 567 WP_011983947.1 signal peptidase I -
  JC776_RS03235 (JC776_03235) - 636326..636919 (-) 594 WP_011983946.1 TVP38/TMEM64 family protein -
  JC776_RS22465 - 636985..637113 (+) 129 WP_275425714.1 hypothetical protein -
  JC776_RS03240 (JC776_03240) - 637192..637782 (-) 591 WP_011983945.1 competence protein ComK -
  JC776_RS03245 (JC776_03245) - 638180..638383 (-) 204 WP_011983944.1 cold shock domain-containing protein -
  JC776_RS03250 (JC776_03250) aceA 638714..639991 (-) 1278 WP_011983943.1 isocitrate lyase -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134531.07 Da        Isoelectric Point: 5.3403

>NTDB_id=518381 JC776_RS03225 WP_048722281.1 632063..635575(-) (addB) [Bacillus cytotoxicus strain E8.1]
MSLRFVIGRAGSGKSTLCLREVQEELKERPRGKTILYLVPEQMTFQTQQALIGSEEVRASIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIIESRKEGLYVFQKAAEQNGFFEHLGGMIAEFKRYNITPSHVYEMWQQLDAHSSSAEQKLLA
NKIYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLPFSEYVKDAEIYIDGFHSFSPQELEIIRQLMRCGARMTITLTVDE
KTFAQPVNELDLFYETVLTYEKVKQVAREEKVVMEKTIPLVEQPRFHSPALAHLEAHYESRPNEKFYGETSIAIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVLRTLFADYDIPHFIDEKRSMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVKKEVMREEMDEFENYCLAYGVQGKKWTADEPWTYRRYRSLDEGNEVITDRERQMEEKMNRLRE
IVRTPIIRLQKRLKRASTVMQMCEAVYLFLEELDVPKKLEQLRVRAEESGDFLFATDHDQVWEEVMNLLDTFVEMLGEES
MSLSVFIDVMTTGLEALQFANIPPSLDQVLIANIDRSRLSNIRATFVIGANEGVMPATPTDEGMLSDEERDVLAAAGVEL
APTTRQTLLEEQFVLYQMVTRASEKLYISCPLADEEGKTLLASSFMKKIKRMFPNVKETFVTNDVNDLSRAAQISYVTTP
EVTLSYVMQQLQTWKRYGFEGDLDFWWDVYNFYVTSDEWKQKSNRVLSSLFYRNRAKKLSTAVSRILYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLREKRTWADLSIKECEHLSILVIEEIAPLLQRQIL
LSSNRHAYLKQKLQQIIFRTSLVLREHAKASGFVPVDLEVPFGMGGKDSLPPMEFTLSNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVISNAGTWMKQGEVASPAGVLYFHIHNPLVEVKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSNIASEQEFSVLQKYVHHTFETIGKDITEGVIDIAP
YKMGNKAACTFCHFRSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=518381 JC776_RS03225 WP_048722281.1 632063..635575(-) (addB) [Bacillus cytotoxicus strain E8.1]
ATGTCACTTCGATTTGTGATTGGAAGAGCTGGAAGTGGGAAAAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
AGAGCGCCCAAGAGGGAAAACAATCTTGTATCTTGTTCCTGAACAAATGACATTTCAGACGCAGCAGGCGTTAATAGGAA
GTGAAGAGGTAAGAGCTTCTATTCGTGCACAAGTGTTTAGTTTTTCACGATTAGCTTGGAAAGTGTTGCAAGAAGTAGGA
GGGGCAAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGCAAAATTATTGAATCGCGCAAAGAAGGATT
ATATGTGTTTCAAAAAGCCGCTGAACAAAATGGCTTCTTTGAACATCTTGGAGGTATGATTGCAGAGTTTAAACGTTACA
ATATAACGCCATCTCATGTATATGAAATGTGGCAACAGTTAGATGCGCATAGCAGTAGCGCCGAACAAAAGTTGCTGGCA
AATAAAATATATGATTTGCAGCTATTATATGATGATTTTGAACGAGCTTTAATTGGAAAGTATTTAGATTCGGAAGATTA
TTTACAGCTCCTTATTGAGAAACTGCCGTTTTCTGAGTATGTAAAGGATGCAGAAATTTATATTGATGGATTTCATTCGT
TCTCCCCGCAAGAGCTTGAAATTATAAGGCAGCTAATGAGATGCGGAGCAAGAATGACGATTACATTAACGGTGGATGAA
AAAACGTTCGCCCAGCCAGTCAATGAACTGGATTTGTTTTATGAAACAGTGCTGACGTATGAAAAAGTAAAACAAGTAGC
ACGTGAAGAGAAAGTGGTAATGGAAAAAACGATTCCGCTCGTAGAGCAGCCGCGATTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCACTATGAATCGCGTCCGAACGAAAAGTTTTACGGAGAGACGAGTATTGCCATCCATACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGAGTAGCGCGAGAAATTCGTAGACTTGTTGCAGAAGAGAATTATCGATACCGCGATATTGCCGT
ACTTCTTCGGAACGGAGAAAGCTATTATGATGTGTTAAGAACATTATTTGCAGATTATGATATTCCGCATTTCATTGATG
AAAAGCGCTCGATGTCTCACCATCCGTTAGTCGAATGCATTCGTTCTGCTTTAGAAATAATTAGTGGGAATTGGCGTTAT
GATGCTGTTTTTCGCTGCGTGAAAACAGAACTTTTATATCCGCTGGATGTAAAAAAAGAAGTGATGCGTGAAGAAATGGA
TGAGTTTGAAAACTACTGTTTGGCGTATGGTGTCCAAGGTAAGAAATGGACCGCCGATGAGCCGTGGACGTATCGTCGTT
ATCGCTCTCTTGATGAGGGGAACGAGGTGATAACAGATCGTGAACGCCAAATGGAAGAGAAAATGAACCGTCTTCGTGAG
ATTGTAAGAACGCCAATCATTCGTCTGCAAAAAAGATTGAAACGTGCAAGTACTGTTATGCAAATGTGCGAAGCTGTATA
TTTATTTTTAGAGGAGCTAGATGTTCCGAAGAAATTAGAACAACTACGTGTACGTGCTGAAGAAAGCGGGGATTTCTTAT
TTGCGACAGACCATGATCAAGTGTGGGAAGAAGTGATGAATCTTCTTGATACATTTGTTGAGATGCTTGGAGAAGAAAGT
ATGTCTCTATCGGTGTTTATTGATGTGATGACGACAGGGCTTGAGGCACTTCAATTTGCCAATATCCCGCCATCGTTAGA
CCAAGTATTAATTGCAAATATCGATCGCTCTAGACTTTCAAATATTCGGGCAACATTTGTGATTGGTGCAAATGAAGGTG
TGATGCCAGCTACGCCGACAGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTGCAGCAGCGGGCGTGGAATTA
GCACCAACGACAAGACAAACGTTATTAGAAGAACAGTTTGTCTTGTATCAAATGGTGACAAGAGCATCTGAGAAACTATA
TATCTCTTGTCCTCTTGCAGATGAAGAAGGAAAGACATTACTAGCTTCTAGTTTTATGAAGAAAATAAAGAGAATGTTTC
CTAATGTGAAAGAGACGTTTGTAACAAACGATGTAAACGACTTATCGCGCGCAGCACAAATTTCATATGTAACGACGCCA
GAAGTAACATTGTCATATGTGATGCAACAGCTGCAAACGTGGAAGCGATACGGTTTTGAAGGTGATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACATCCGATGAATGGAAGCAAAAAAGCAACCGTGTATTATCCAGTTTGTTTTATCGAA
ATCGTGCGAAGAAATTAAGTACGGCTGTAAGTCGAATTTTATATGGAGATAAAATAAAAGGAAGTGTCTCTCGTATGGAA
TTATTTAATCGCTGTGCGTACGCTCATTTTGCACAGCACGGTTTATCATTGAGAGAGCGCGATATATTTAAATTAGATGC
ACCAGATATCGGGGAGCTGTTCCATGCAGCGCTAAAGAAAATTGCGGACAAGCTCTTGCGTGAAAAACGGACTTGGGCTG
ATTTATCCATAAAAGAATGTGAGCATCTTTCCATATTAGTAATAGAAGAAATTGCTCCATTATTACAAAGGCAAATTTTA
TTAAGCTCAAATCGACATGCTTATTTGAAACAAAAATTACAGCAAATTATTTTTAGAACATCGCTTGTGCTTAGGGAACA
TGCTAAGGCGAGTGGATTTGTTCCAGTTGATTTAGAAGTTCCGTTTGGTATGGGAGGCAAAGACTCATTACCACCGATGG
AATTTACATTATCAAACGGTGTGAAAATGGAAGTTGTCGGCCGGATTGATCGAGTTGATAAAGCGGAAGATGAGAGCGGT
ACGTTTCTTCGCATTATTGATTATAAATCGAGCTCGAAAGCGCTGGATTTAACAGAAGTATATTACGGATTAGCATTGCA
AATGCTGACGTATTTGGATGTCGTCATTTCTAATGCTGGTACGTGGATGAAACAGGGGGAAGTCGCATCACCAGCAGGTG
TGTTATACTTCCATATTCATAATCCACTTGTTGAGGTGAAAGGTGACGCTTCTGAAGAGGAGATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTGCTTGGAGATGCAGATGTTGTTCGTTTAATGGATAACAAACTATCAACAGGAAG
CTCTGACATTATTTCAGCCGGGTTAAAAAAAGACGGAAGCTTCAGCGCACGCTCTAACATCGCAAGTGAACAAGAATTTT
CTGTGCTACAAAAATACGTACACCATACGTTTGAAACGATCGGAAAAGACATTACAGAAGGTGTCATTGATATTGCTCCA
TATAAAATGGGGAATAAAGCAGCGTGCACATTTTGTCACTTCCGCTCTGTCTGCCAGTTCGATGAATCGCTGGAAGACAA
TCAGTTCCGTACATTAAAAGATATGAAAGATAGTGAAGCAATGGAGAAAATACGAGAGGAGGTCGGGGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.53

100

0.496