Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KZO18_RS12650 Genome accession   NZ_CP080085
Coordinates   2560183..2560860 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 19SZHZ869Rt     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2555183..2565860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZO18_RS12615 (KZO18_12590) gspG 2555663..2556100 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  KZO18_RS12620 (KZO18_12595) gspH 2556108..2556617 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KZO18_RS12625 (KZO18_12600) gspI 2556614..2556991 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KZO18_RS12630 (KZO18_12605) gspJ 2556984..2557571 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KZO18_RS12635 (KZO18_12610) gspK 2557564..2558547 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  KZO18_RS12640 (KZO18_12615) gspL 2558562..2559725 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  KZO18_RS12645 (KZO18_12620) gspM 2559722..2560183 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  KZO18_RS12650 (KZO18_12625) pilD 2560183..2560860 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KZO18_RS12655 (KZO18_12630) bfr 2560889..2561365 (-) 477 WP_000675504.1 bacterioferritin -
  KZO18_RS12660 (KZO18_12635) bfd 2561437..2561631 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KZO18_RS12665 (KZO18_12640) chiA 2561800..2564493 (-) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=516946 KZO18_RS12650 WP_000178161.1 2560183..2560860(+) (pilD) [Escherichia coli strain 19SZHZ869Rt]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=516946 KZO18_RS12650 WP_000178161.1 2560183..2560860(+) (pilD) [Escherichia coli strain 19SZHZ869Rt]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378