NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	I6H87_RS09550	Genome accession	NZ_CP066018
Coordinates	2046649..2047563 (-)	Length	304 a.a.
NCBI ID	WP_011616083.1	Uniprot ID	Q0K6P0
Organism	Cupriavidus necator strain FDAARGOS_1030
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2041649..2052563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6H87_RS09525 (I6H87_09525)	-	2042175..2042621 (+)	447	WP_011616085.1	NUDIX domain-containing protein	-
I6H87_RS09530 (I6H87_09530)	-	2042715..2044754 (+)	2040	WP_010814756.1	OPT family oligopeptide transporter	-
I6H87_RS09535 (I6H87_09535)	-	2044785..2044973 (-)	189	WP_010814755.1	DNA gyrase inhibitor YacG	-
I6H87_RS09540 (I6H87_09540)	zapD	2044991..2045749 (-)	759	WP_011616084.1	cell division protein ZapD	-
I6H87_RS09545 (I6H87_09545)	coaE	2045959..2046585 (-)	627	WP_010814753.1	dephospho-CoA kinase	-
I6H87_RS09550 (I6H87_09550)	pilD	2046649..2047563 (-)	915	WP_011616083.1	prepilin peptidase	Machinery gene
I6H87_RS09555 (I6H87_09555)	-	2047567..2048838 (-)	1272	WP_011616082.1	type II secretion system F family protein	-
I6H87_RS09560 (I6H87_09560)	pilF	2048903..2050627 (-)	1725	WP_011616081.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I6H87_RS09565 (I6H87_09565)	-	2051051..2051371 (+)	321	WP_062804630.1	helix-turn-helix domain-containing protein	-

Sequence

Protein

Download Length: 304 a.a. Molecular weight: 32407.54 Da Isoelectric Point: 7.6360

                                >NTDB_id=516416 I6H87_RS09550 WP_011616083.1 2046649..2047563(-) (pilD) [Cupriavidus necator strain FDAARGOS_1030]

MLSSWSASPYPSPASTGGAPLVEALAALPPAFLVAAAALLGLVVGSFLNVVIHRMPRMMERDEANYIAELRGDPLPYPGR

YNLMVPRSACPHCGHAIAPWENVPVLSYLFLRGRCSACQAPISVRYPLVELACGVLSALAAWRFGPGGQALAALVLVWAL

LALTMIDADTQLLPDQITLPLLWLGLLLNLGGVFVTLPDAVIGAAAGYLVLWTAYWLFRLVRGKEGMGFGDFKLMAALGA

WLGWQALPALVLLSSVVGVLFGLANIALRRQERDTPFPFGPFIALAGVVVLLFGPGVLPLFAWP

Nucleotide

Download Length: 915 bp

                                >NTDB_id=516416 I6H87_RS09550 WP_011616083.1 2046649..2047563(-) (pilD) [Cupriavidus necator strain FDAARGOS_1030]

ATGTTGTCTTCGTGGTCTGCTTCTCCCTACCCTTCGCCGGCCTCCACCGGCGGCGCGCCGCTGGTGGAGGCACTGGCGGC

GTTGCCGCCCGCCTTCCTGGTGGCGGCCGCCGCGCTGCTCGGGCTGGTGGTCGGCAGCTTCCTCAATGTGGTCATCCACC

GGATGCCGCGCATGATGGAGCGCGACGAGGCCAACTATATCGCCGAGCTGCGCGGCGACCCGCTGCCCTACCCCGGCCGC

TACAACCTGATGGTGCCGCGCTCGGCCTGCCCGCATTGCGGCCACGCGATTGCGCCGTGGGAGAACGTGCCGGTGCTGAG

CTACCTGTTCCTGCGCGGGCGCTGCTCGGCGTGCCAGGCGCCGATCAGTGTGCGCTATCCGCTGGTCGAGCTGGCCTGTG

GCGTGCTGAGCGCGCTGGCGGCGTGGCGCTTCGGCCCCGGCGGACAGGCCCTGGCCGCGCTGGTGCTGGTGTGGGCGCTG

CTGGCGCTGACCATGATCGATGCCGATACGCAACTGCTGCCGGACCAGATCACCCTGCCGCTGTTGTGGCTGGGCCTGCT

GCTGAACCTGGGCGGCGTGTTCGTGACGCTGCCCGACGCCGTGATCGGCGCGGCTGCCGGCTACCTGGTGCTGTGGACGG

CCTACTGGCTGTTCCGGCTGGTGCGCGGCAAGGAAGGCATGGGCTTTGGCGACTTCAAGCTGATGGCGGCGCTGGGCGCC

TGGCTCGGCTGGCAGGCGCTGCCGGCGCTGGTGCTGCTGTCGTCGGTGGTCGGCGTGCTGTTCGGGCTGGCCAATATCGC

GCTGCGCCGGCAGGAACGCGACACGCCCTTCCCGTTCGGGCCCTTCATCGCGCTCGCCGGCGTGGTGGTGCTGCTGTTCG

GCCCCGGCGTGCTGCCGCTGTTTGCATGGCCATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(39-144)

Type IV leader peptidase family (Peptidase_A24)

(154-263)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q0K6P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	55.849	87.171	0.487
pilD	Vibrio campbellii strain DS40M4	52.19	90.132	0.47
pilD	Neisseria gonorrhoeae MS11	47.887	93.421	0.447
pilD	Acinetobacter baumannii D1279779	47.601	89.145	0.424
pilD	Acinetobacter nosocomialis M2	47.232	89.145	0.421

Detailed information NTG0516416