Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   I6H57_RS01795 Genome accession   NZ_CP065991
Coordinates   372208..372750 (+) Length   180 a.a.
NCBI ID   WP_005695588.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain FDAARGOS_1000     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 367208..377750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H57_RS01775 (I6H57_01775) - 367881..368777 (-) 897 WP_005695582.1 manganese/iron ABC transporter ATP-binding protein -
  I6H57_RS01780 (I6H57_01780) - 368777..369658 (-) 882 WP_005695583.1 metal ABC transporter substrate-binding protein -
  I6H57_RS01785 (I6H57_01785) - 369813..370688 (-) 876 WP_005695584.1 D-alanyl-D-alanine carboxypeptidase family protein -
  I6H57_RS01790 (I6H57_01790) - 370990..372138 (+) 1149 WP_005695586.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  I6H57_RS01795 (I6H57_01795) pilF/pilF2 372208..372750 (+) 543 WP_005695588.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6H57_RS01800 (I6H57_01800) - 372824..373852 (+) 1029 WP_005695589.1 RodZ family helix-turn-helix domain-containing protein -
  I6H57_RS01805 (I6H57_01805) ispG 373871..374977 (+) 1107 WP_005695590.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6H57_RS01810 (I6H57_01810) hisS 374987..376258 (+) 1272 WP_005695591.1 histidine--tRNA ligase -
  I6H57_RS01815 (I6H57_01815) - 376277..376891 (+) 615 WP_005695592.1 YfgM family protein -
  I6H57_RS01820 (I6H57_01820) - 376943..377386 (+) 444 WP_005695593.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20343.01 Da        Isoelectric Point: 6.4774

>NTDB_id=516024 I6H57_RS01795 WP_005695588.1 372208..372750(+) (pilF/pilF2) [Haemophilus parainfluenzae strain FDAARGOS_1000]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKALEHDERYYLVHSALAHFYQ
LQGDPEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=516024 I6H57_RS01795 WP_005695588.1 372208..372750(+) (pilF/pilF2) [Haemophilus parainfluenzae strain FDAARGOS_1000]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTCTCTCAGTCTTCCGT
TGACTTTAATAAACAACAAGCAGCAAAAGCGCGAGTTGAACTGGCATTAGGCTATCTTCAACAAAATGATTTTGTTCAAG
CAAAACTCAATTTAGATAAAGCCCTTGAGCATGATGAACGATATTATCTTGTGCACTCAGCACTTGCACATTTTTATCAA
TTACAAGGCGATCCGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTCGGTGCTTTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCACTCGCTGCGCCGA
ATTATTACCATCAAGCCGATACTTACGAAAACATGGCGCTTTGTGCTTTCGCCGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(71-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.279

95.556

0.633
  pilF Glaesserella parasuis strain SC1401

49.718

98.333

0.489