Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   I8N73_RS18745 Genome accession   NZ_CP065791
Coordinates   3803890..3805035 (+) Length   381 a.a.
NCBI ID   WP_080130556.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM279     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3798890..3810035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N73_RS18725 (I8N73_18725) - 3799787..3800215 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  I8N73_RS18730 (I8N73_18730) - 3800369..3800881 (+) 513 Protein_3659 bacteriocin biosynthesis protein AlbG -
  I8N73_RS18735 (I8N73_18735) - 3800888..3802264 (-) 1377 WP_199022226.1 YncE family protein -
  I8N73_RS18740 (I8N73_18740) - 3802304..3803659 (-) 1356 WP_199022227.1 YncE family protein -
  I8N73_RS18745 (I8N73_18745) rapF 3803890..3805035 (+) 1146 WP_080130556.1 tetratricopeptide repeat protein Regulator
  I8N73_RS18750 (I8N73_18750) phrF 3805019..3805138 (+) 120 WP_041482476.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  I8N73_RS18755 (I8N73_18755) - 3805336..3805539 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  I8N73_RS18760 (I8N73_18760) - 3805553..3805756 (+) 204 WP_007407641.1 hypothetical protein -
  I8N73_RS18765 (I8N73_18765) - 3805791..3805976 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  I8N73_RS18770 (I8N73_18770) - 3806162..3806443 (+) 282 WP_045209208.1 hypothetical protein -
  I8N73_RS18775 (I8N73_18775) - 3806487..3806999 (+) 513 WP_199022228.1 stage II sporulation protein M -
  I8N73_RS18780 (I8N73_18780) - 3807590..3807808 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  I8N73_RS18785 (I8N73_18785) speB 3807976..3808848 (-) 873 WP_007614448.1 agmatinase -
  I8N73_RS18790 (I8N73_18790) speE 3808907..3809737 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45615.74 Da        Isoelectric Point: 4.5769

>NTDB_id=514580 I8N73_RS18745 WP_080130556.1 3803890..3805035(+) (rapF) [Bacillus velezensis strain LBUM279]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=514580 I8N73_RS18745 WP_080130556.1 3803890..3805035(+) (rapF) [Bacillus velezensis strain LBUM279]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCAGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCCTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567