Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6G27_RS03155 Genome accession   NZ_CP065751
Coordinates   701130..702005 (+) Length   291 a.a.
NCBI ID   WP_062332387.1    Uniprot ID   A0A378Q6Z4
Organism   Moraxella osloensis strain FDAARGOS_870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 696130..707005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G27_RS03135 (I6G27_03135) secG 696439..696759 (-) 321 WP_062332397.1 preprotein translocase subunit SecG -
  I6G27_RS03140 (I6G27_03140) tpiA 696850..697704 (-) 855 WP_062332394.1 triose-phosphate isomerase -
  I6G27_RS03145 (I6G27_03145) pilB 698138..699844 (+) 1707 WP_062332391.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G27_RS03150 (I6G27_03150) pilC 699901..701124 (+) 1224 WP_062332389.1 type II secretion system F family protein Machinery gene
  I6G27_RS03155 (I6G27_03155) pilD 701130..702005 (+) 876 WP_062332387.1 A24 family peptidase Machinery gene
  I6G27_RS03160 (I6G27_03160) coaE 702002..702658 (+) 657 WP_227713376.1 dephospho-CoA kinase -
  I6G27_RS03165 (I6G27_03165) - 702716..704122 (-) 1407 WP_062332386.1 nicotinate phosphoribosyltransferase -
  I6G27_RS03170 (I6G27_03170) - 704191..705294 (-) 1104 WP_082741424.1 bifunctional nicotinamide-nucleotide adenylyltransferase/Nudix hydroxylase -
  I6G27_RS03175 (I6G27_03175) nfuA 705592..706209 (+) 618 WP_062332384.1 Fe-S biogenesis protein NfuA -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32289.78 Da        Isoelectric Point: 8.1744

>NTDB_id=513966 I6G27_RS03155 WP_062332387.1 701130..702005(+) (pilD) [Moraxella osloensis strain FDAARGOS_870]
MGIFSDFILLLQQSQWLTLIIVALLGLCVGSFLNVVIYRTPLMMEQQWKNECQLLLHPETIVDHHESITLSRPVSRCPHC
GHSIRWFENMPVISWLLLKGKCSQCHHAISYRYPLVEIITAILSVLVVMKFGVTWQALAGLVLTWTLVALTGIDFDTQLL
PDRFTLPLAGLGLFVNAFGLFVSPTAAIFGYVFGFLCLWIVYQLFLLITGKHGMGHGDFKLLAALGAWLGPLMLPLIVLL
SAFVGSVVGVILMKRSGESKPFAFGPYIAIAGMIALLYGQSIMSWYLGKMS

Nucleotide


Download         Length: 876 bp        

>NTDB_id=513966 I6G27_RS03155 WP_062332387.1 701130..702005(+) (pilD) [Moraxella osloensis strain FDAARGOS_870]
ATGGGGATTTTTTCAGATTTTATTTTGTTGTTACAACAGTCACAATGGCTGACGTTGATTATCGTAGCTTTATTGGGATT
ATGCGTGGGCAGTTTTCTCAATGTGGTGATTTATCGCACGCCTTTGATGATGGAGCAGCAGTGGAAGAACGAATGCCAGC
TATTGCTTCATCCAGAAACCATTGTTGACCATCATGAATCCATTACCTTAAGCCGTCCTGTTTCTCGTTGTCCGCATTGT
GGGCATTCAATACGTTGGTTTGAAAATATGCCTGTTATTAGCTGGCTACTGCTAAAAGGAAAATGTAGTCAGTGTCATCA
CGCCATTAGTTATCGTTATCCGCTTGTAGAAATCATCACCGCTATTTTGTCTGTGCTGGTGGTGATGAAATTTGGTGTTA
CTTGGCAAGCGCTGGCAGGATTGGTACTCACATGGACATTAGTGGCTTTGACAGGCATTGATTTTGATACCCAATTGTTG
CCTGACCGATTTACATTGCCGTTAGCGGGATTAGGCTTATTTGTCAATGCCTTTGGCTTGTTTGTCAGTCCCACAGCGGC
TATTTTTGGCTATGTGTTTGGGTTTTTATGCTTGTGGATTGTCTACCAACTGTTTTTGCTGATTACGGGCAAGCACGGGA
TGGGGCATGGCGATTTTAAATTATTAGCGGCTTTGGGGGCTTGGCTAGGGCCTTTGATGCTACCGTTAATTGTGCTTTTA
TCTGCATTTGTCGGCTCGGTTGTGGGTGTAATATTGATGAAGCGCTCAGGTGAGAGTAAACCTTTTGCTTTTGGACCCTA
TATCGCCATTGCTGGCATGATCGCACTTTTGTATGGGCAATCAATAATGTCGTGGTATCTAGGCAAAATGTCATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(24-131)

Type IV leader peptidase family (Peptidase_A24)

(141-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378Q6Z4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

63.028

97.595

0.615
  pilD Acinetobacter nosocomialis M2

63.028

97.595

0.615
  pilD Vibrio campbellii strain DS40M4

48.754

96.564

0.471
  pilD Vibrio cholerae strain A1552

49.27

94.158

0.464
  pilD Neisseria gonorrhoeae MS11

45.185

92.784

0.419