Detailed information NTG0513921

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I6G47_RS28280
• Genome accession: NZ_CP065748
• Coordinates: 6080291..6081184 (-)
• Length: 297 a.a.
• NCBI ID: WP_016445609.1
• Uniprot ID: A0A1H3L211
• Organism: Delftia lacustris strain FDAARGOS_890
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6075291..6086184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G47_RS28250 (I6G47_28250)	argJ	6075617..6076849 (+)	1233	WP_016451021.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
I6G47_RS28255 (I6G47_28255)	-	6077015..6077971 (+)	957	WP_016451022.1	ATP-binding protein	-
I6G47_RS28260 (I6G47_28260)	-	6078022..6078528 (+)	507	WP_016445605.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
I6G47_RS28265 (I6G47_28265)	-	6078539..6078793 (-)	255	WP_016451023.1	DNA gyrase inhibitor YacG	-
I6G47_RS28270 (I6G47_28270)	zapD	6078793..6079548 (-)	756	WP_016445607.1	cell division protein ZapD	-
I6G47_RS28275 (I6G47_28275)	coaE	6079638..6080276 (-)	639	WP_016451024.1	dephospho-CoA kinase	-
I6G47_RS28280 (I6G47_28280)	pilD	6080291..6081184 (-)	894	WP_016445609.1	prepilin peptidase	Machinery gene
I6G47_RS28285 (I6G47_28285)	pilC	6081184..6082401 (-)	1218	WP_016445610.1	type II secretion system F family protein	Machinery gene
I6G47_RS28290 (I6G47_28290)	pilB	6082440..6084182 (-)	1743	WP_016445611.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I6G47_RS28300 (I6G47_28300)	-	6084419..6085348 (-)	930	WP_016451025.1	polyprenyl synthetase family protein	-
I6G47_RS28305 (I6G47_28305)	rplU	6085579..6085890 (+)	312	WP_012207284.1	50S ribosomal protein L21	-
I6G47_RS28310 (I6G47_28310)	rpmA	6085906..6086163 (+)	258	WP_012207283.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31842.37 Da
• Isoelectric Point: 6.9135

>NTDB_id=513921 I6G47_RS28280 WP_016445609.1 6080291..6081184(-) (pilD) [Delftia lacustris strain FDAARGOS_890]
MVLELWMQAALGGLLGLLVGSFLNVVVHRLPRMMEQQWNAECAQWAQEQSSSSATPSPATAPPPAEAPLSLSRPRSRCPH
CGHAIHWYENIPVLSYLALRGRCSECKASISIRYPLVELACAALFAYCAAQWGITPTGAAWCLFSAALLSLALIDWDTTL
LPDDITLPLLWAGLLSSALHWTAVPLADAVWGAAAGYLSLWLVYWAFKLATGKEGMGYGDFKLFAALGAWFGWQALVPII
LISSVIGAIVGIALKINSRLREGGYVPFGPFLAGAGFAAMVFGPQQMLQSLLAALGL

Nucleotide

• Length: 894 bp

>NTDB_id=513921 I6G47_RS28280 WP_016445609.1 6080291..6081184(-) (pilD) [Delftia lacustris strain FDAARGOS_890]
ATGGTTTTGGAACTCTGGATGCAGGCTGCATTGGGCGGCCTGCTGGGCCTGTTGGTTGGCAGCTTTCTCAACGTGGTGGT
GCATAGGCTGCCCCGCATGATGGAACAGCAATGGAATGCGGAATGCGCCCAATGGGCGCAAGAGCAATCTTCATCCTCCG
CCACGCCATCGCCCGCCACAGCGCCGCCGCCTGCCGAGGCGCCCCTGAGCCTGAGCCGCCCCCGCTCGCGCTGCCCGCAT
TGCGGCCATGCCATCCACTGGTACGAGAATATTCCCGTGCTCAGTTATCTGGCGCTGCGAGGCCGGTGCTCCGAATGCAA
GGCGTCGATCAGCATCCGCTATCCGCTGGTGGAATTGGCCTGCGCCGCGCTGTTTGCCTACTGTGCGGCGCAATGGGGCA
TCACGCCCACAGGCGCTGCCTGGTGCCTTTTCAGTGCGGCCTTGCTGAGCCTGGCATTGATCGACTGGGACACCACACTG
CTTCCCGACGACATCACCCTGCCCTTGCTGTGGGCCGGACTGCTGTCGTCGGCGCTGCACTGGACCGCCGTGCCTCTGGC
CGACGCGGTATGGGGCGCTGCAGCGGGCTATCTCTCGCTGTGGCTGGTTTACTGGGCCTTCAAGCTGGCCACCGGCAAGG
AAGGCATGGGCTACGGCGACTTCAAGCTGTTTGCCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCACTCGTGCCCATCATC
CTGATCTCGTCGGTGATTGGCGCCATCGTGGGCATCGCGTTGAAGATCAACAGCCGCCTGCGTGAGGGCGGCTATGTTCC
CTTCGGCCCCTTTCTGGCGGGCGCCGGCTTTGCAGCCATGGTGTTCGGACCGCAGCAGATGCTGCAATCCCTATTGGCGG
CCTTGGGCCTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-131)

• Type IV leader peptidase family (Peptidase_A24) (143-252)

3D structure

• AlphaFold DB: A0A1H3L211

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.59	95.286	0.492
pilD	Vibrio campbellii strain DS40M4	46.416	98.653	0.458
pilD	Acinetobacter nosocomialis M2	44.364	92.593	0.411
pilD	Neisseria gonorrhoeae MS11	45.865	89.562	0.411
pilD	Acinetobacter baumannii D1279779	44	92.593	0.407