NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	I6G23_RS05320	Genome accession	NZ_CP065735
Coordinates	772209..772856 (+)	Length	215 a.a.
NCBI ID	WP_010906131.1	Uniprot ID	A0A3N6KQH1
Organism	Lactococcus lactis strain FDAARGOS_866
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 767209..777856

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G23_RS05295 (I6G23_05295)	-	767338..768516 (+)	1179	WP_021214596.1	SLC13 family permease	-
I6G23_RS05300 (I6G23_05300)	-	768689..769252 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
I6G23_RS05305 (I6G23_05305)	-	769356..769754 (+)	399	WP_004255236.1	hypothetical protein	-
I6G23_RS05310 (I6G23_05310)	-	769859..770791 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
I6G23_RS05315 (I6G23_05315)	-	770788..772149 (+)	1362	WP_021214597.1	ABC transporter permease	-
I6G23_RS05320 (I6G23_05320)	comEA	772209..772856 (+)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
I6G23_RS05325 (I6G23_05325)	comEC	772837..775047 (+)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I6G23_RS05330 (I6G23_05330)	-	775332..776108 (+)	777	WP_004255244.1	alpha/beta hydrolase	-
I6G23_RS05335 (I6G23_05335)	-	776292..776507 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
I6G23_RS05340 (I6G23_05340)	atpB	776552..777265 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
I6G23_RS05345 (I6G23_05345)	atpF	777280..777786 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23624.76 Da Isoelectric Point: 4.8659

                                >NTDB_id=513675 I6G23_RS05320 WP_010906131.1 772209..772856(+) (comEA) [Lactococcus lactis strain FDAARGOS_866]

MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=513675 I6G23_RS05320 WP_010906131.1 772209..772856(+) (comEA) [Lactococcus lactis strain FDAARGOS_866]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A3N6KQH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386

Detailed information NTG0513675