Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I6G25_RS08705 Genome accession   NZ_CP065729
Coordinates   1644612..1645973 (-) Length   453 a.a.
NCBI ID   WP_015912383.1    Uniprot ID   B9E8S3
Organism   Macrococcoides caseolyticum strain FDAARGOS_868     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1639612..1650973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G25_RS08680 (I6G25_08655) cysE 1640038..1640691 (-) 654 WP_101038220.1 serine O-acetyltransferase -
  I6G25_RS08685 (I6G25_08660) gltX 1640929..1642383 (-) 1455 WP_103214807.1 glutamate--tRNA ligase -
  I6G25_RS08690 (I6G25_08665) ispF 1642401..1642880 (-) 480 WP_086039304.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  I6G25_RS08695 (I6G25_08670) ispD 1642877..1643557 (-) 681 WP_086039305.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I6G25_RS08700 (I6G25_08675) - 1643550..1644599 (-) 1050 WP_101141328.1 PIN/TRAM domain-containing protein -
  I6G25_RS08705 (I6G25_08680) radA 1644612..1645973 (-) 1362 WP_015912383.1 DNA repair protein RadA Machinery gene
  I6G25_RS08710 (I6G25_08685) - 1646196..1648622 (-) 2427 WP_101141329.1 ATP-dependent Clp protease ATP-binding subunit -
  I6G25_RS08715 (I6G25_08690) - 1648632..1649636 (-) 1005 WP_101141331.1 protein arginine kinase -
  I6G25_RS08720 (I6G25_08695) - 1649626..1650012 (-) 387 WP_015912387.1 UvrB/UvrC motif-containing protein -
  I6G25_RS08725 (I6G25_08700) - 1650023..1650484 (-) 462 WP_101141334.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50240.85 Da        Isoelectric Point: 7.2379

>NTDB_id=513642 I6G25_RS08705 WP_015912383.1 1644612..1645973(-) (radA) [Macrococcoides caseolyticum strain FDAARGOS_868]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSDNKVEKLKDITKESVPRDHTQMKELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV
KRSKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP
RRMASGVDHNRLNLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKMAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=513642 I6G25_RS08705 WP_015912383.1 1644612..1645973(-) (radA) [Macrococcoides caseolyticum strain FDAARGOS_868]
TTGGCAAAGGTGAAAAGTACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGAAAATGTCCGAATTG
TGGTGCATGGAATCAAATGGAAGAAGTTATTGAACATAAACAGAAGGGTCCTAAAAATGCGATTTCTGAATCACAAAGTG
ATAATAAAGTTGAAAAACTAAAAGATATAACGAAAGAAAGTGTTCCTCGTGATCACACGCAGATGAAGGAACTAGATCGT
GTACTTGGAGGAGGAATCGTTCCCGGTTCATTGATACTTATCGGCGGAGACCCAGGGATTGGGAAATCCACATTACTACT
ACAAGTTTGTGCAATGCTTTCACAAAACCATCCCGTACTTTATATTTCTGGAGAGGAATCGGTAAGACAAACGAAACTAC
GTGCCGATCGTCTATTAGAGGATGCAGGGGAGCTTGATGTCTATGCAGAAACCAACCTTCAGATTATTCACGAAACGGTA
AAACGATCGAAACCGAAATTTTTAGTTATCGATTCAATTCAAACAATATTTCATCCAGAGGTTACAAGTGCACCTGGTTC
TGTATCGCAAGTTAGAGAATGTACACAAGAGTTAATGCGTATCGCAAAGCAGATGAACATTGCAACATTTATCGTAGGGC
ACGTAACAAAAGAAGGACAAATTGCAGGTCCAAGGCTACTAGAACATATGGTTGATACAGTTCTTTACTTTGAAGGAGAC
ACTCATCACAGCTACAGAATATTACGCGCGGTGAAGAATCGTTTTGGTTCGACAAATGAAATGGGTATATTTGAAATGAA
GAATACAGGCCTTAAAGAAGTCCTGAATCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCAGGTTCAACTA
TCGTTGCAACGATGGAAGGCACGCGTCCATTACTAGTTGAAGTTCAGTCACTTGTAACGCCGACATCTTTTCATAATCCA
AGAAGAATGGCATCAGGTGTCGACCATAATCGACTGAACCTATTGATGGCTGTACTAGAGAAAAAACAAGGATATTTATT
ACAGCAACAGGATGCATATGTGAAAGTTGCGGGTGGTGTAAAACTTGATGAACCTGCTGTTGATTTAAGTGTTATCGTAA
GCATCGCTTCAAGCTATAATGATAAACCGACACGAGGCGATGACTGTTTTATCGGAGAAGTCGGTCTGACTGGTGAAGTG
AGACGTGTTGCACGTATTGAACAACGTGTACAAGAAGCTGAAAAACTCGGTTTTAAACGTGTCATCATCCCAAAAAATAA
TATTGGTGGCTGGGATTTCCCCGGAAATATTGAAGTGATTGGTGTAACAAATATTAACGAAGCATTAAAAATGGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E8S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.447

100

0.669
  radA Streptococcus pneumoniae Rx1

62.996

100

0.631
  radA Streptococcus pneumoniae D39

62.996

100

0.631
  radA Streptococcus pneumoniae R6

62.996

100

0.631
  radA Streptococcus pneumoniae TIGR4

62.996

100

0.631
  radA Streptococcus mitis NCTC 12261

62.996

100

0.631
  radA Streptococcus mitis SK321

62.996

100

0.631