Detailed information NTG0513599

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I6G28_RS08325
• Genome accession: NZ_CP065726
• Coordinates: 1671806..1672666 (+)
• Length: 286 a.a.
• NCBI ID: WP_111726191.1
• Uniprot ID: A0A7T3ESH3
• Organism: Neisseria cinerea strain FDAARGOS_871
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1666806..1677666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G28_RS08310 (I6G28_08310)	dapD	1667468..1668289 (-)	822	WP_003676582.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
I6G28_RS08315 (I6G28_08315)	pgi	1668499..1670142 (-)	1644	WP_111726193.1	glucose-6-phosphate isomerase	-
I6G28_RS08320 (I6G28_08320)	pilG	1670500..1671732 (+)	1233	WP_107996268.1	type II secretion system F family protein	Machinery gene
I6G28_RS08325 (I6G28_08325)	pilD	1671806..1672666 (+)	861	WP_111726191.1	prepilin peptidase	Machinery gene
I6G28_RS08330 (I6G28_08330)	coaE	1672668..1673300 (+)	633	WP_111726190.1	dephospho-CoA kinase	-
I6G28_RS08335 (I6G28_08335)	-	1673293..1673502 (+)	210	WP_039854241.1	DNA gyrase inhibitor YacG	-
I6G28_RS08340 (I6G28_08340)	pilB	1673595..1675270 (-)	1676	Protein_1607	type IV-A pilus assembly ATPase PilB	-
I6G28_RS08350 (I6G28_08350)	-	1675620..1676066 (-)	447	WP_003676595.1	DUF441 domain-containing protein	-
I6G28_RS08355 (I6G28_08355)	-	1676071..1677045 (-)	975	WP_108043479.1	polyprenyl synthetase family protein	-
I6G28_RS08360 (I6G28_08360)	rplU	1677268..1677576 (+)	309	WP_002216394.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31256.25 Da
• Isoelectric Point: 8.4715

>NTDB_id=513599 I6G28_RS08325 WP_111726191.1 1671806..1672666(+) (pilD) [Neisseria cinerea strain FDAARGOS_871]
MSDLSILSAFVVPLAAVFGLLVGSFLNVVIYRIPVMMERGWTVFAKEHLNLPLTDEESHTFNLMKPDSCCPKCRAPVRAW
QNIPIVSYLFLRGKCASCRTKISIRYPLIELLTGILFGLVAWQYGWSWITLGGLILTAFLISLTLIDADTQYLPDSMTLP
LIWLGLLFNFNGDFVSLQSAVSGAVAGYGSLWLLCAVYKLLTGKIGMGNGDFKLLAALGAWLGISVLPVLVFLAALVGLA
MAVIMRISKGQYFAFGPALAISGWIIFAANDSVLRAVNWWLTHSGL

Nucleotide

• Length: 861 bp

>NTDB_id=513599 I6G28_RS08325 WP_111726191.1 1671806..1672666(+) (pilD) [Neisseria cinerea strain FDAARGOS_871]
ATGTCTGATTTGTCTATATTGTCGGCATTTGTCGTACCGTTGGCAGCGGTGTTCGGGCTGCTGGTTGGAAGTTTCTTAAA
CGTCGTCATTTACCGCATACCGGTCATGATGGAGCGCGGTTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGATGAGGAAAGCCATACCTTCAACCTGATGAAACCGGATTCCTGTTGCCCGAAATGTCGCGCACCGGTACGCGCATGG
CAGAATATTCCGATTGTCAGCTACCTATTCCTGCGCGGAAAATGTGCCTCCTGCCGAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCATATTGTTCGGCCTGGTCGCATGGCAATACGGCTGGTCTTGGATTACACTGGGCGGTT
TGATACTGACCGCATTTTTGATTTCCCTTACCCTCATCGATGCAGATACCCAATATCTGCCCGATTCAATGACCCTACCC
TTAATTTGGCTGGGGCTGCTGTTCAACTTTAACGGGGATTTCGTGTCTTTACAATCGGCGGTTTCAGGCGCGGTTGCCGG
CTACGGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTTACAGGAAAAATCGGCATGGGTAACGGCGACTTCAAAC
TGCTTGCCGCATTAGGGGCGTGGCTCGGCATATCCGTACTACCCGTTCTGGTATTCCTTGCCGCACTGGTCGGCTTGGCA
ATGGCAGTCATCATGCGCATCTCCAAAGGGCAATATTTTGCCTTCGGTCCCGCACTGGCAATCTCAGGCTGGATTATCTT
CGCAGCAAACGATTCCGTACTTCGTGCGGTTAATTGGTGGCTTACCCATTCGGGACTGTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: A0A7T3ESH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	85.866	98.951	0.85
pilD	Acinetobacter baumannii D1279779	46.619	98.252	0.458
pilD	Vibrio cholerae strain A1552	47.636	96.154	0.458
pilD	Vibrio campbellii strain DS40M4	46.403	97.203	0.451
pilD	Acinetobacter nosocomialis M2	48.485	92.308	0.448