Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   I6H05_RS05490 Genome accession   NZ_CP065604
Coordinates   1166987..1167538 (+) Length   183 a.a.
NCBI ID   WP_025298068.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain FDAARGOS_948     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1161987..1172538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H05_RS05475 (I6H05_05475) - 1162937..1164301 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  I6H05_RS05480 (I6H05_05480) ansB 1164482..1165531 (-) 1050 WP_005568022.1 L-asparaginase 2 -
  I6H05_RS05485 (I6H05_05485) - 1165805..1166965 (+) 1161 WP_155266098.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  I6H05_RS05490 (I6H05_05490) pilF/pilF2 1166987..1167538 (+) 552 WP_025298068.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6H05_RS05495 (I6H05_05495) - 1167682..1168737 (+) 1056 WP_005568019.1 helix-turn-helix domain-containing protein -
  I6H05_RS05500 (I6H05_05500) ispG 1168749..1169852 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6H05_RS05505 (I6H05_05505) hisS 1169874..1171151 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  I6H05_RS05510 (I6H05_05510) - 1171162..1171776 (+) 615 WP_025298069.1 YfgM family protein -
  I6H05_RS05515 (I6H05_05515) - 1171829..1172281 (+) 453 WP_005568016.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20697.48 Da        Isoelectric Point: 8.8869

>NTDB_id=512178 I6H05_RS05490 WP_025298068.1 1166987..1167538(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain FDAARGOS_948]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTTYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=512178 I6H05_RS05490 WP_025298068.1 1166987..1167538(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain FDAARGOS_948]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCACCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

46.471

92.896

0.432