Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I4W80_RS11300 Genome accession   NZ_CP065591
Coordinates   2216266..2217336 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain 1817     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2211266..2222336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I4W80_RS11250 (I4W80_11250) sipW 2211361..2211933 (-) 573 WP_017357979.1 signal peptidase I SipW -
  I4W80_RS11255 (I4W80_11255) tapA 2211983..2212510 (-) 528 WP_017357980.1 amyloid fiber anchoring/assembly protein TapA -
  I4W80_RS11260 (I4W80_11260) - 2212781..2213089 (+) 309 WP_206116235.1 DUF3889 domain-containing protein -
  I4W80_RS11265 (I4W80_11265) - 2213124..2213318 (-) 195 WP_008342191.1 YqzE family protein -
  I4W80_RS11270 (I4W80_11270) - 2213379..2213762 (-) 384 WP_017357982.1 competence type IV pilus minor pilin ComGG -
  I4W80_RS11275 (I4W80_11275) comGF 2213759..2214256 (-) 498 WP_017357983.1 competence type IV pilus minor pilin ComGF -
  I4W80_RS11280 (I4W80_11280) comGE 2214198..2214512 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  I4W80_RS11285 (I4W80_11285) comGD 2214496..2214942 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  I4W80_RS11290 (I4W80_11290) comGC 2214935..2215228 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  I4W80_RS11295 (I4W80_11295) comGB 2215245..2216285 (-) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  I4W80_RS11300 (I4W80_11300) comGA 2216266..2217336 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  I4W80_RS11305 (I4W80_11305) - 2217802..2218671 (-) 870 WP_007501231.1 STAS domain-containing protein -
  I4W80_RS11315 (I4W80_11315) - 2218897..2219277 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  I4W80_RS11320 (I4W80_11320) - 2219490..2219732 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  I4W80_RS11325 (I4W80_11325) - 2219781..2220419 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  I4W80_RS11330 (I4W80_11330) - 2220580..2220753 (+) 174 WP_017357990.1 DUF2759 domain-containing protein -
  I4W80_RS11335 (I4W80_11335) - 2220977..2221291 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=512125 I4W80_RS11300 WP_017357987.1 2216266..2217336(-) (comGA) [Bacillus altitudinis strain 1817]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=512125 I4W80_RS11300 WP_017357987.1 2216266..2217336(-) (comGA) [Bacillus altitudinis strain 1817]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTTTCTACATTACCGACTGTAAATGATGAAAGTTTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATCCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGTGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649