Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGD   Type   Machinery gene
Locus tag   I5367_RS11660 Genome accession   NZ_CP065539
Coordinates   2427218..2427655 (-) Length   145 a.a.
NCBI ID   WP_012117983.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2422218..2432655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS11610 (I5367_11610) sinI 2422602..2422775 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  I5367_RS11615 (I5367_11615) sinR 2422809..2423144 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  I5367_RS11620 (I5367_11620) tasA 2423192..2423977 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  I5367_RS11625 (I5367_11625) sipW 2424042..2424626 (-) 585 WP_012117977.1 signal peptidase I SipW -
  I5367_RS11630 (I5367_11630) tapA 2424598..2425269 (-) 672 WP_012117978.1 amyloid fiber anchoring/assembly protein TapA -
  I5367_RS11635 (I5367_11635) - 2425528..2425857 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  I5367_RS11640 (I5367_11640) - 2425897..2426076 (-) 180 WP_003153093.1 YqzE family protein -
  I5367_RS11645 (I5367_11645) comGG 2426133..2426510 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  I5367_RS11650 (I5367_11650) comGF 2426511..2427011 (-) 501 WP_012117981.1 competence type IV pilus minor pilin ComGF -
  I5367_RS11655 (I5367_11655) comGE 2426920..2427234 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  I5367_RS11660 (I5367_11660) comGD 2427218..2427655 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  I5367_RS11665 (I5367_11665) comGC 2427645..2427953 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  I5367_RS11670 (I5367_11670) comGB 2427958..2428995 (-) 1038 WP_012117984.1 competence type IV pilus assembly protein ComGB Machinery gene
  I5367_RS11675 (I5367_11675) comGA 2428982..2430052 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  I5367_RS11680 (I5367_11680) - 2430249..2431199 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  I5367_RS11685 (I5367_11685) - 2431345..2432646 (+) 1302 WP_012117986.1 hemolysin family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16286.74 Da        Isoelectric Point: 10.2475

>NTDB_id=511768 I5367_RS11660 WP_012117983.1 2427218..2427655(-) (comGD) [Bacillus amyloliquefaciens strain DGL1]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPAYTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=511768 I5367_RS11660 WP_012117983.1 2427218..2427655(-) (comGD) [Bacillus amyloliquefaciens strain DGL1]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGCCTACACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGACTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACGGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.849

100

0.572