Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ST4147_RS07235 Genome accession   NZ_CP065504
Coordinates   1388367..1389062 (-) Length   231 a.a.
NCBI ID   WP_011226400.1    Uniprot ID   Q5M374
Organism   Streptococcus thermophilus strain 4147     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1383367..1394062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4147_RS07220 (ST4147_07235) - 1384178..1384723 (-) 546 WP_022096992.1 GNAT family N-acetyltransferase -
  ST4147_RS07225 (ST4147_07240) - 1384789..1386051 (-) 1263 WP_041827084.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ST4147_RS07230 (ST4147_07245) - 1386137..1388377 (-) 2241 WP_011227470.1 DNA internalization-related competence protein ComEC/Rec2 -
  ST4147_RS07235 (ST4147_07250) comEA 1388367..1389062 (-) 696 WP_011226400.1 helix-hairpin-helix domain-containing protein Machinery gene
  ST4147_RS07240 (ST4147_07255) - 1389170..1389925 (-) 756 WP_002948862.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  ST4147_RS07245 - 1390416..1390648 (+) 233 Protein_1373 polysaccharide deacetylase family protein -
  ST4147_RS07250 - 1390703..1390990 (+) 288 WP_011226402.1 peptidoglycan GlcNAc deacetylase -
  ST4147_RS07255 (ST4147_07265) - 1391040..1391804 (+) 765 WP_011226403.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ST4147_RS07260 (ST4147_07270) - 1391808..1392077 (+) 270 WP_011226404.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 24466.40 Da        Isoelectric Point: 4.6300

>NTDB_id=511331 ST4147_RS07235 WP_011226400.1 1388367..1389062(-) (comEA) [Streptococcus thermophilus strain 4147]
MKEKILAYVKDNRLFVSVIAVLMVIFCFFLWMTCGAGNSMEAETSYTDVTALSTSSSKQSSQSLSEASSQSKTEGSEKVK
SKVTVDVKGAVVNPGVYTLKAGARVTDVIQEAGGITEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQTGTGQVSDKG
GQAVSKDGKINLNTATSEQLQTISGIGAKRAEDIIAYRESHGGFQSVDDLKNVSGIGDKTLDKIRESLYVA

Nucleotide


Download         Length: 696 bp        

>NTDB_id=511331 ST4147_RS07235 WP_011226400.1 1388367..1389062(-) (comEA) [Streptococcus thermophilus strain 4147]
GTGAAGGAAAAGATTCTAGCCTATGTCAAAGATAATCGTCTGTTTGTGAGTGTCATCGCTGTACTGATGGTGATTTTTTG
CTTCTTTTTATGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCAGAGACGTCTTATACAGATGTGACAGCTTTGTCAA
CGTCTTCATCCAAACAAAGTTCACAGTCTCTTTCTGAGGCGTCTTCCCAGTCAAAAACCGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAATCCAGGTGTCTATACGCTAAAGGCAGGCGCTAGGGTGACAGA
TGTCATTCAGGAAGCTGGAGGAATTACAGAAGATGCAGACGCTAAGAGTGTTAACTTAGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGATGAAAATGTTTCTGTCCTTGATCAAACAGGTACTGGTCAGGTCTCTGACAAAGGG
GGGCAGGCTGTATCTAAGGATGGCAAAATTAACTTAAATACAGCGACCTCAGAACAGTTGCAAACCATTTCTGGAATTGG
AGCTAAGCGGGCAGAGGATATCATTGCCTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGATGACTTGAAAAATGTCT
CAGGAATTGGTGATAAAACATTAGATAAAATCAGAGAGTCCCTCTATGTGGCTTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(85-138)

Helix-hairpin-helix motif (HHH_3)

(167-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M374

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

99.134

100

0.991
  comEA/celA/cilE Streptococcus mitis SK321

41.048

99.134

0.407
  comEA/celA/cilE Streptococcus pneumoniae D39

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae R6

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae Rx1

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

39.474

98.701

0.39
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.158

98.701

0.377