Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ST4147_RS01975 Genome accession   NZ_CP065504
Coordinates   359015..360334 (+) Length   439 a.a.
NCBI ID   WP_011226886.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain 4147     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354015..365334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4147_RS01950 (ST4147_01945) - 354869..355633 (+) 765 WP_002947187.1 ABC transporter ATP-binding protein -
  ST4147_RS01955 (ST4147_01950) - 355633..356343 (+) 711 WP_002947183.1 ABC transporter ATP-binding protein -
  ST4147_RS01960 (ST4147_01955) - 356479..357139 (+) 661 Protein_334 CBS and ACT domain-containing protein -
  ST4147_RS01965 (ST4147_01960) cysK 357306..358232 (-) 927 WP_002947179.1 cysteine synthase A -
  ST4147_RS01970 (ST4147_01965) - 358334..358960 (-) 627 WP_002949745.1 YigZ family protein -
  ST4147_RS01975 (ST4147_01970) comFA/cflA 359015..360334 (+) 1320 WP_011226886.1 DEAD/DEAH box helicase Machinery gene
  ST4147_RS01980 (ST4147_01975) - 360315..360977 (+) 663 WP_002949747.1 ComF family protein -
  ST4147_RS01985 (ST4147_01980) raiA 361056..361604 (+) 549 WP_011225518.1 ribosome-associated translation inhibitor RaiA -
  ST4147_RS01990 (ST4147_01985) - 361634..362587 (-) 954 WP_041826943.1 IS30 family transposase -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50654.75 Da        Isoelectric Point: 9.5504

>NTDB_id=511306 ST4147_RS01975 WP_011226886.1 359015..360334(+) (comFA/cflA) [Streptococcus thermophilus strain 4147]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKYHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHYLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCREEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=511306 ST4147_RS01975 WP_011226886.1 359015..360334(+) (comFA/cflA) [Streptococcus thermophilus strain 4147]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGTATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATTACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGGAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae D39

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae R6

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae TIGR4

54.988

98.178

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

54.651

97.95

0.535
  comFA/cflA Streptococcus mitis SK321

53.721

97.95

0.526
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367