Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   STMM20_RS08170 Genome accession   NZ_CP065485
Coordinates   1607814..1609133 (-) Length   439 a.a.
NCBI ID   WP_014607993.1    Uniprot ID   A0AAN1ZSQ9
Organism   Streptococcus thermophilus strain MM20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1602814..1614133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STMM20_RS08160 (STMM20_08130) raiA 1606544..1607092 (-) 549 WP_011680772.1 ribosome-associated translation inhibitor RaiA -
  STMM20_RS08165 (STMM20_08135) - 1607171..1607833 (-) 663 WP_014607994.1 ComF family protein -
  STMM20_RS08170 (STMM20_08140) comFA/cflA 1607814..1609133 (-) 1320 WP_014607993.1 DEAD/DEAH box helicase Machinery gene
  STMM20_RS08175 (STMM20_08145) - 1609188..1609814 (+) 627 WP_011225515.1 YigZ family protein -
  STMM20_RS08180 (STMM20_08150) cysK 1609916..1610842 (+) 927 WP_014607992.1 cysteine synthase A -
  STMM20_RS08185 (STMM20_08155) - 1611009..1611669 (-) 661 Protein_1572 CBS and ACT domain-containing protein -
  STMM20_RS08190 (STMM20_08160) - 1611805..1612515 (-) 711 WP_011680767.1 ABC transporter ATP-binding protein -
  STMM20_RS08195 (STMM20_08165) - 1612515..1613279 (-) 765 WP_002949740.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50524.63 Da        Isoelectric Point: 9.5841

>NTDB_id=510497 STMM20_RS08170 WP_014607993.1 1607814..1609133(-) (comFA/cflA) [Streptococcus thermophilus strain MM20]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPCFFWQGRLQKSLTSDKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFSQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=510497 STMM20_RS08170 WP_014607993.1 1607814..1609133(-) (comFA/cflA) [Streptococcus thermophilus strain MM20]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTTGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGATAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTTCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-206)

Helicase conserved C-terminal domain (Helicase_C)

(290-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae Rx1

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae D39

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae R6

54.988

98.178

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

54.651

97.95

0.535
  comFA/cflA Streptococcus mitis SK321

53.721

97.95

0.526
  comFA Lactococcus lactis subsp. cremoris KW2

46.19

95.672

0.442
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367