Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I3X05_RS01405 Genome accession   NZ_CP065217
Coordinates   322298..323317 (+) Length   339 a.a.
NCBI ID   WP_045569760.1    Uniprot ID   A0A0F2I9E8
Organism   Vibrio navarrensis strain 20-VB00237     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317298..328317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X05_RS01390 (I3X05_01385) - 318243..318500 (-) 258 WP_045569763.1 DUF3624 domain-containing protein -
  I3X05_RS01395 (I3X05_01390) oxyR 318671..319564 (+) 894 WP_045569762.1 DNA-binding transcriptional regulator OxyR -
  I3X05_RS01400 (I3X05_01395) - 319643..322159 (-) 2517 WP_045569761.1 PBP1A family penicillin-binding protein -
  I3X05_RS01405 (I3X05_01400) pilM 322298..323317 (+) 1020 WP_045569760.1 type IV pilus assembly protein PilM Machinery gene
  I3X05_RS01410 (I3X05_01405) pilN 323301..323888 (+) 588 WP_045569759.1 PilN domain-containing protein Machinery gene
  I3X05_RS01415 (I3X05_01410) pilO 323881..324471 (+) 591 WP_045569758.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3X05_RS01420 (I3X05_01415) pilP 324461..324973 (+) 513 WP_045569757.1 pilus assembly protein PilP Machinery gene
  I3X05_RS01425 (I3X05_01420) pilQ 325004..326779 (+) 1776 WP_045569756.1 type IV pilus secretin PilQ family protein Machinery gene
  I3X05_RS01430 (I3X05_01425) aroK 326989..327507 (+) 519 WP_045569755.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38185.97 Da        Isoelectric Point: 7.6101

>NTDB_id=508182 I3X05_RS01405 WP_045569760.1 322298..323317(+) (pilM) [Vibrio navarrensis strain 20-VB00237]
MGKPLVTGIDIGHRSLKAVILKPAGNSYALLGYKEIVFERAIVAENHTLNHQEIVNTLKVLNKELPRFRRQVALAVPDSA
VISKQLQIEPIEDEKECEIAILQAFSHQSPFPVDELNIDYVRLHEPAQSKSASQSVQIFATKKEVVESRVRAARAVRLKP
VLVDMHAHCLFNLRDLAAQRYPDKSAFCLLDVGAQSTSLIIHQREKTHFIKEFAYGVEHLNAAIGDEFSAHDAQEKTERF
IVEMVDRVARQLQLFQSINSQDKPQGLWLCGEGAATPLLLEALSRRLSLECELLNPLGLFEMKVARKKRRSVDWHHFSVA
AGLAIGGIQWLENKHEAHH

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=508182 I3X05_RS01405 WP_045569760.1 322298..323317(+) (pilM) [Vibrio navarrensis strain 20-VB00237]
ATGGGTAAACCCTTAGTTACCGGGATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCCGCAGGAAATAG
CTATGCCTTGCTGGGATATAAAGAAATTGTATTTGAGCGCGCTATTGTCGCCGAAAACCACACTCTGAATCATCAGGAAA
TTGTCAATACGCTTAAAGTCCTGAACAAAGAGTTGCCGCGTTTCCGTCGTCAGGTGGCGCTGGCCGTGCCGGATAGTGCC
GTGATCAGCAAGCAATTGCAAATTGAACCGATAGAAGATGAGAAAGAGTGTGAAATCGCCATTTTGCAGGCTTTTTCCCA
TCAATCGCCATTTCCGGTCGATGAGCTGAATATTGATTATGTGCGCTTGCATGAACCCGCGCAGAGTAAAAGCGCCAGCC
AGAGCGTGCAGATCTTTGCCACCAAAAAAGAGGTGGTCGAGAGTCGGGTGCGCGCTGCGCGAGCGGTGAGGTTGAAACCG
GTGTTGGTGGATATGCACGCCCACTGCTTGTTTAACCTGCGAGATCTGGCGGCGCAGCGATACCCAGACAAAAGTGCTTT
TTGTTTGTTGGATGTCGGTGCGCAATCCACCTCGCTGATTATTCATCAGCGCGAGAAAACGCACTTCATCAAAGAGTTTG
CATACGGCGTTGAGCACCTGAACGCTGCGATTGGCGATGAGTTCAGTGCCCATGATGCGCAAGAGAAAACCGAGCGCTTT
ATAGTGGAGATGGTGGATCGCGTCGCGCGTCAGTTGCAGCTCTTTCAGTCCATCAATAGCCAAGACAAGCCACAAGGGCT
TTGGTTGTGTGGCGAGGGAGCGGCGACGCCTCTGTTGCTCGAGGCGCTCTCTCGTCGTTTGTCGTTGGAGTGCGAACTGC
TCAATCCGCTCGGTCTGTTTGAGATGAAAGTGGCACGCAAAAAACGTCGCAGTGTTGACTGGCACCACTTTTCGGTCGCC
GCTGGGCTAGCCATCGGTGGGATTCAATGGTTGGAGAACAAACATGAAGCACACCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2I9E8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

54.572

100

0.546
  pilM Vibrio cholerae strain A1552

47.536

100

0.484