Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I3X05_RS01415 Genome accession   NZ_CP065217
Coordinates   323881..324471 (+) Length   196 a.a.
NCBI ID   WP_045569758.1    Uniprot ID   A0A0F2I700
Organism   Vibrio navarrensis strain 20-VB00237     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318881..329471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X05_RS01400 (I3X05_01395) - 319643..322159 (-) 2517 WP_045569761.1 PBP1A family penicillin-binding protein -
  I3X05_RS01405 (I3X05_01400) pilM 322298..323317 (+) 1020 WP_045569760.1 type IV pilus assembly protein PilM Machinery gene
  I3X05_RS01410 (I3X05_01405) pilN 323301..323888 (+) 588 WP_045569759.1 PilN domain-containing protein Machinery gene
  I3X05_RS01415 (I3X05_01410) pilO 323881..324471 (+) 591 WP_045569758.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3X05_RS01420 (I3X05_01415) pilP 324461..324973 (+) 513 WP_045569757.1 pilus assembly protein PilP Machinery gene
  I3X05_RS01425 (I3X05_01420) pilQ 325004..326779 (+) 1776 WP_045569756.1 type IV pilus secretin PilQ family protein Machinery gene
  I3X05_RS01430 (I3X05_01425) aroK 326989..327507 (+) 519 WP_045569755.1 shikimate kinase AroK -
  I3X05_RS01435 (I3X05_01430) aroB 327540..328631 (+) 1092 WP_045569754.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22729.99 Da        Isoelectric Point: 4.3595

>NTDB_id=508184 I3X05_RS01415 WP_045569758.1 323881..324471(+) (pilO) [Vibrio navarrensis strain 20-VB00237]
MVDWQDLDIDEIAEWPLLPQSLVVLLLMMIIQGFGYWFYLQPEQEALQRAVEEEQNLKVALRIKANKAAALPQLQSQLDE
LSKRYDYLLQQLPAQKELATLLASVNELGLENDLTFTRIDWGEKLSKDFLYQLPLNIELTGNYNDIGQFSQAIAELPRII
SFSDVTWQRVSLESSTLHFRVKANTYQFKPEVTHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=508184 I3X05_RS01415 WP_045569758.1 323881..324471(+) (pilO) [Vibrio navarrensis strain 20-VB00237]
ATGGTTGATTGGCAGGATCTCGATATCGACGAAATTGCGGAGTGGCCATTGTTGCCACAAAGCTTGGTGGTGCTACTACT
GATGATGATCATTCAGGGCTTTGGCTACTGGTTCTATTTGCAACCCGAGCAAGAGGCGTTGCAACGGGCGGTGGAAGAGG
AGCAAAACCTCAAAGTCGCGCTGCGCATCAAAGCCAACAAAGCGGCCGCCTTGCCACAGCTTCAGTCTCAGTTGGATGAG
TTGTCAAAACGCTACGACTATTTGCTCCAGCAACTGCCCGCGCAAAAGGAGCTAGCGACCTTGCTCGCGTCGGTCAATGA
ACTCGGGCTTGAAAACGATCTCACTTTTACCCGCATCGATTGGGGGGAAAAACTGAGCAAGGACTTCCTATATCAGCTCC
CTCTCAACATTGAACTGACCGGGAATTACAACGACATCGGCCAGTTTTCCCAAGCGATCGCCGAGCTGCCGCGCATCATT
AGCTTTAGCGACGTCACTTGGCAGCGGGTCAGTTTGGAAAGCAGCACACTGCATTTTCGCGTTAAAGCCAACACGTACCA
GTTTAAGCCGGAGGTGACCCATGAAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2I700

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

75.789

96.939

0.735
  pilO Vibrio cholerae strain A1552

68.367

100

0.684