Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I3X05_RS01425 Genome accession   NZ_CP065217
Coordinates   325004..326779 (+) Length   591 a.a.
NCBI ID   WP_045569756.1    Uniprot ID   A0A0F2IBH2
Organism   Vibrio navarrensis strain 20-VB00237     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320004..331779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X05_RS01405 (I3X05_01400) pilM 322298..323317 (+) 1020 WP_045569760.1 type IV pilus assembly protein PilM Machinery gene
  I3X05_RS01410 (I3X05_01405) pilN 323301..323888 (+) 588 WP_045569759.1 PilN domain-containing protein Machinery gene
  I3X05_RS01415 (I3X05_01410) pilO 323881..324471 (+) 591 WP_045569758.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3X05_RS01420 (I3X05_01415) pilP 324461..324973 (+) 513 WP_045569757.1 pilus assembly protein PilP Machinery gene
  I3X05_RS01425 (I3X05_01420) pilQ 325004..326779 (+) 1776 WP_045569756.1 type IV pilus secretin PilQ family protein Machinery gene
  I3X05_RS01430 (I3X05_01425) aroK 326989..327507 (+) 519 WP_045569755.1 shikimate kinase AroK -
  I3X05_RS01435 (I3X05_01430) aroB 327540..328631 (+) 1092 WP_045569754.1 3-dehydroquinate synthase -
  I3X05_RS01440 (I3X05_01435) - 328683..330188 (+) 1506 WP_045569753.1 AAA family ATPase -
  I3X05_RS01445 (I3X05_01440) - 330268..331095 (+) 828 WP_045569752.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 64027.06 Da        Isoelectric Point: 4.7438

>NTDB_id=508186 I3X05_RS01425 WP_045569756.1 325004..326779(+) (pilQ) [Vibrio navarrensis strain 20-VB00237]
MTMKKGMIRLQAPLRQLMCLFAVCLFATFANAQSENNGQSASENALKGIDFRTNQNKAAVIIVELASPAAVVDLKPVQEG
LSIELLNTRVSDEQLYLLDVKDFSTPVESVEVFRDVSATRLLASVSGEFHHEYRLTGRYLEVTVSRIETPQQVEEKSILE
KEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKEELD
LREKQHLEKQRLAEELGTLKSEIIKVNFAKASDIAEMISGTESVSMLSERGSIAIDERTNSLLLRELPENIAVIREIVNS
LDIPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSHTVGGSIENNLASIGLYNAAGGGSGSGGDSGGGSSSIDDFLNV
NLGATSGNASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSFKKA
VLSLKVTPQITPDNRLVLDLSVTQDRPGEVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITSTVDKVPLLGD
LPLLGALFRRSYENIGKRELLIFVTPKVVIQ

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=508186 I3X05_RS01425 WP_045569756.1 325004..326779(+) (pilQ) [Vibrio navarrensis strain 20-VB00237]
ATGACCATGAAGAAAGGAATGATTCGATTACAGGCTCCGCTACGCCAATTAATGTGCCTGTTTGCTGTCTGCCTCTTTGC
CACGTTTGCAAACGCGCAGTCAGAGAATAACGGCCAGTCAGCGAGTGAGAATGCGCTGAAAGGCATTGATTTCAGAACCA
ACCAGAATAAAGCGGCAGTAATTATTGTCGAGTTGGCTTCGCCTGCCGCGGTGGTGGATCTTAAACCTGTGCAGGAGGGG
CTCAGCATTGAGCTGCTCAACACCCGCGTCAGCGACGAACAGCTCTATCTATTAGACGTAAAGGACTTCTCGACGCCGGT
TGAAAGTGTTGAAGTGTTCCGCGATGTGTCAGCGACGCGGTTGTTGGCGTCTGTCTCGGGGGAGTTTCACCATGAGTATC
GTTTGACTGGGCGCTATTTGGAAGTGACGGTCAGTCGCATAGAAACTCCGCAGCAAGTTGAGGAGAAAAGCATCCTTGAA
AAAGAGGGCAAGCTTATCTCGATCAACTTCCAAGACATTCCGGTGAGGAACGTCTTGCAACTGATAGCGGACTACAACGG
TTTTAATCTAGTGGTCTCCGATTCGGTCACGGGCAATCTGACGCTGCGTTTGGATGGCGTGCCGTGGCAGCAAGTACTCG
ACATCATTTTGCAAGTCAAAGGGCTCGACAAGCGGGTGGAAGGCAATGTGGTGCTGATCGCGCCAAAAGAAGAGTTGGAT
TTGCGTGAAAAGCAGCATCTGGAAAAGCAGCGTTTGGCGGAAGAGCTAGGCACGCTTAAATCGGAAATCATCAAGGTGAA
TTTTGCCAAAGCGTCGGACATTGCCGAGATGATCAGCGGCACGGAATCGGTCAGCATGCTGTCCGAGCGCGGCAGCATTG
CGATTGATGAACGCACGAACTCCTTGCTACTACGAGAACTGCCGGAAAACATTGCAGTGATTCGTGAGATTGTCAATTCG
CTCGATATTCCGGTCAAGCAGGTGCAAATTGAGGCGCGTATTGTCACCGTCAATGAAGGTAATTTGGATGAACTGGGCGT
ACGCTGGGGCTTCACCTCGATCAACGGTAGCCACACGGTGGGCGGTTCGATCGAGAACAACTTGGCGAGCATCGGTTTGT
ACAATGCGGCCGGTGGCGGTTCTGGCTCAGGAGGCGATAGCGGTGGCGGCTCTAGCAGTATCGACGATTTTCTCAACGTC
AACCTCGGTGCAACCTCCGGCAATGCATCGAGCATCGCTTTCCAGGTCGCCAAGCTGGGTTCGGATCTGCTGCTTGATTT
GGAACTGTCGGCGCTGCAGCGAGAGTCAAAAGCGGAGATCATTTCCAGCCCACGTTTGATCACCACCAATAAAAAGCCCG
CCTACATCGAACAAGGGACCGAAATTCCCTATCTGGAATCGTCCTCCAGTGGCGCGACCACCGTCTCGTTTAAAAAGGCG
GTGCTGAGTTTGAAAGTCACACCACAGATCACCCCAGATAACCGTTTGGTGCTCGATCTTAGCGTCACGCAAGACAGGCC
GGGTGAAGTGGTGAAAACGGGCACGGGTGAAGCGGTAGCGATTGACACCCAACGTATCGGCACTCAGGTGTTGGTTAACA
ATGGTGAAACCGTGGTTCTCGGCGGCATTTTCCAACACAGCATCACCAGTACGGTCGACAAAGTGCCTTTGCTGGGCGAC
CTACCGTTGCTCGGCGCGCTGTTTCGCCGTAGCTATGAAAATATCGGTAAACGGGAACTGTTGATCTTTGTGACGCCAAA
GGTGGTTATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(262-327)

Bacterial type II and III secretion system protein (Secretin)

(431-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2IBH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

78.511

100

0.785
  pilQ Vibrio cholerae strain A1552

78.739

93.909

0.739
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.739

93.909

0.739
  pilQ Pseudomonas aeruginosa PAK

40.468

94.078

0.381
  pilQ Legionella pneumophila strain ERS1305867

39.964

93.57

0.374
  pilQ Legionella pneumophila strain Lp02

39.964

93.57

0.374
  comQ Acinetobacter baylyi ADP1

38.169

97.97

0.374
  pilQ Acinetobacter baumannii strain A118

36.486

100

0.365
  pilQ Acinetobacter baumannii D1279779

36.486

100

0.365