Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I3X02_RS16135 Genome accession   NZ_CP065213
Coordinates   3162612..3163034 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain HGS7     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3157612..3168034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X02_RS16110 - 3157940..3158773 (-) 834 WP_209367036.1 AraC family transcriptional regulator -
  I3X02_RS16115 - 3158994..3159815 (+) 822 WP_140451350.1 histidine kinase -
  I3X02_RS16120 - 3159816..3160457 (+) 642 WP_140451349.1 response regulator transcription factor -
  I3X02_RS16125 - 3160595..3161977 (+) 1383 WP_209367038.1 PepSY domain-containing protein -
  I3X02_RS16130 - 3162007..3162471 (+) 465 WP_014459243.1 FixH family protein -
  I3X02_RS16135 nucA/comI 3162612..3163034 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  I3X02_RS16140 - 3163175..3163528 (+) 354 WP_226701378.1 DUF1516 family protein -
  I3X02_RS16145 - 3163569..3164162 (-) 594 WP_115654367.1 hypothetical protein -
  I3X02_RS16150 - 3164316..3164972 (-) 657 WP_014459240.1 TetR/AcrR family transcriptional regulator -
  I3X02_RS16155 - 3164996..3166273 (-) 1278 WP_209367040.1 glycosyltransferase -
  I3X02_RS16160 - 3166588..3167133 (+) 546 WP_209367042.1 GNAT family N-acetyltransferase -
  I3X02_RS16165 - 3167265..3167750 (-) 486 WP_209367044.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=508147 I3X02_RS16135 WP_013057949.1 3162612..3163034(+) (nucA/comI) [Priestia megaterium strain HGS7]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=508147 I3X02_RS16135 WP_013057949.1 3162612..3163034(+) (nucA/comI) [Priestia megaterium strain HGS7]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGTTCTTGGATTTCACATCAAGTAGATGAGTTTCCAGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507