Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IG605_RS03500 Genome accession   NZ_CP065178
Coordinates   750064..751104 (+) Length   346 a.a.
NCBI ID   WP_193407882.1    Uniprot ID   -
Organism   Pectobacterium quasiaquaticum strain A398-S21-F17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745064..756104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG605_RS03465 (IG605_003455) - 745575..745697 (+) 123 Protein_662 GNAT family N-acetyltransferase -
  IG605_RS03470 (IG605_003460) hemW 745773..746912 (-) 1140 WP_193407885.1 radical SAM family heme chaperone HemW -
  IG605_RS03475 (IG605_003465) - 746905..747498 (-) 594 WP_193407884.1 XTP/dITP diphosphatase -
  IG605_RS03480 (IG605_003470) yggU 747516..747806 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  IG605_RS03485 (IG605_003475) - 747803..748357 (-) 555 WP_010295003.1 YggT family protein -
  IG605_RS03490 (IG605_003480) proC 748407..749228 (-) 822 WP_193397469.1 pyrroline-5-carboxylate reductase -
  IG605_RS03495 (IG605_003485) - 749330..750043 (-) 714 WP_193407883.1 YggS family pyridoxal phosphate-dependent enzyme -
  IG605_RS03500 (IG605_003490) pilT 750064..751104 (+) 1041 WP_193407882.1 type IV pilus twitching motility protein PilT Machinery gene
  IG605_RS03505 (IG605_003495) - 751106..752677 (-) 1572 WP_193407881.1 methyl-accepting chemotaxis protein -
  IG605_RS03510 (IG605_003500) nsrR 753005..753430 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  IG605_RS03515 (IG605_003505) rnr 753472..755928 (+) 2457 WP_193407880.1 ribonuclease R -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37643.93 Da        Isoelectric Point: 7.0925

>NTDB_id=507709 IG605_RS03500 WP_193407882.1 750064..751104(+) (pilT) [Pectobacterium quasiaquaticum strain A398-S21-F17]
MELDEWMARSVKHNASDLHLCSGHPPMLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLEALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSRDRHVITLE
DPIEFIHASRRCLIQQREIGTHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTASAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHRFSAGPTTGGAIHP

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=507709 IG605_RS03500 WP_193407882.1 750064..751104(+) (pilT) [Pectobacterium quasiaquaticum strain A398-S21-F17]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGAT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACCTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTTCGGCAGGCTGGTCAGGTGGACGGCGCGTTAATGCTGCCTGATGGGCAA
CGCTTGCGGGTGAACGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTGTTATCCCGTCCGTTCAGCCTTCATT
GGAAGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAGCCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCTACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCCGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTACGCACAGCGCATCGTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCTGATGTCATTCTGCTAGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCTCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGCTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGAGTGCGGCAGTCAGCAACTTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAGACAGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACCGTTTTAGCGCTGGGCCGACCACAGGCGGTGCAATCCATCCGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

95.376

0.497
  pilT Vibrio cholerae strain A1552

52.121

95.376

0.497
  pilT Neisseria meningitidis 8013

51.205

95.954

0.491
  pilT Neisseria gonorrhoeae MS11

50.904

95.954

0.488
  pilT Acinetobacter baylyi ADP1

50.151

95.665

0.48
  pilT Pseudomonas stutzeri DSM 10701

49.849

95.665

0.477
  pilT Legionella pneumophila strain Lp02

49.849

95.665

0.477
  pilT Legionella pneumophila strain ERS1305867

49.849

95.665

0.477
  pilT Acinetobacter nosocomialis M2

49.849

95.665

0.477
  pilT Acinetobacter baumannii D1279779

49.849

95.665

0.477
  pilT Acinetobacter baumannii strain A118

49.849

95.665

0.477
  pilT Pseudomonas aeruginosa PAK

49.547

95.665

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.342

96.243

0.408
  pilU Vibrio cholerae strain A1552

39.759

95.954

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.039

96.243

0.376