Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   NT08PM_RS06835 Genome accession   NC_017764
Coordinates   1460759..1461304 (-) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. multocida str. 3480     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1455759..1466304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT08PM_RS06810 (NT08PM_1395) - 1455969..1456406 (-) 438 WP_010907449.1 methyltransferase family protein -
  NT08PM_RS06815 (NT08PM_1396) - 1456619..1457230 (-) 612 WP_005725137.1 YfgM family protein -
  NT08PM_RS06820 (NT08PM_1397) hisS 1457249..1458520 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  NT08PM_RS06825 (NT08PM_1398) ispG 1458550..1459653 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NT08PM_RS06830 (NT08PM_1399) - 1459680..1460636 (-) 957 WP_014391405.1 helix-turn-helix domain-containing protein -
  NT08PM_RS06835 (NT08PM_1400) pilF/pilF2 1460759..1461304 (-) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NT08PM_RS06840 (NT08PM_1401) - 1461343..1462527 (-) 1185 WP_014668288.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NT08PM_RS06845 (NT08PM_1402) - 1462707..1463960 (-) 1254 WP_014391407.1 AI-2E family transporter -
  NT08PM_RS06850 (NT08PM_1403) glnB 1463961..1464299 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  NT08PM_RS06855 (NT08PM_1404) mog 1464344..1464922 (-) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  NT08PM_RS06860 (NT08PM_1405) - 1464948..1465877 (-) 930 WP_014391409.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=50711 NT08PM_RS06835 WP_014391406.1 1460759..1461304(-) (pilF/pilF2) [Pasteurella multocida subsp. multocida str. 3480]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=50711 NT08PM_RS06835 WP_014391406.1 1460759..1461304(-) (pilF/pilF2) [Pasteurella multocida subsp. multocida str. 3480]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47

Multiple sequence alignment