Detailed information NTG0505928

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F131LOC_RS14475
• Genome accession: NZ_CP065030
• Coordinates: 3248304..3249149 (-)
• Length: 281 a.a.
• NCBI ID: WP_103971572.1
• Uniprot ID: A0A855MBX9
• Organism: Pectobacterium versatile strain F131
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3243304..3254149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F131LOC_RS14450 (F131LOC_014450)	-	3243465..3244235 (-)	771	WP_103971570.1	ABC transporter ATP-binding protein	-
F131LOC_RS14455 (F131LOC_014455)	-	3244237..3245274 (-)	1038	WP_039492234.1	FecCD family ABC transporter permease	-
F131LOC_RS14460 (F131LOC_014460)	-	3245271..3246335 (-)	1065	WP_103862228.1	ABC transporter substrate-binding protein	-
F131LOC_RS14465 (F131LOC_014465)	-	3246687..3247712 (+)	1026	WP_103971571.1	LLM class flavin-dependent oxidoreductase	-
F131LOC_RS14470 (F131LOC_014470)	ypdK	3248069..3248143 (+)	75	WP_099606229.1	membrane protein YpdK	-
F131LOC_RS14475 (F131LOC_014475)	pilD	3248304..3249149 (-)	846	WP_103971572.1	prepilin peptidase	Machinery gene
F131LOC_RS14480 (F131LOC_014480)	-	3249239..3249985 (-)	747	WP_103971573.1	type II secretion system protein N	-
F131LOC_RS14485 (F131LOC_014485)	gspM	3249975..3250469 (-)	495	WP_103971574.1	type II secretion system protein GspM	-
F131LOC_RS14490 (F131LOC_014490)	gspL	3250466..3251743 (-)	1278	WP_103971575.1	type II secretion system protein GspL	-
F131LOC_RS14495 (F131LOC_014495)	gspK	3251740..3252726 (-)	987	WP_010302966.1	type II secretion system minor pseudopilin GspK	-
F131LOC_RS14500 (F131LOC_014500)	gspJ	3252723..3253373 (-)	651	WP_103971576.1	type II secretion system minor pseudopilin GspJ	-
F131LOC_RS14505 (F131LOC_014505)	gspI	3253373..3253738 (-)	366	WP_103971577.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31247.14 Da
• Isoelectric Point: 7.0813

>NTDB_id=505928 F131LOC_RS14475 WP_103971572.1 3248304..3249149(-) (pilD) [Pectobacterium versatile strain F131]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDRDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSAQYPLVEVITMLAFLAAGLLWLPGMALLGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=505928 F131LOC_RS14475 WP_103971572.1 3248304..3249149(-) (pilD) [Pectobacterium versatile strain F131]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCAGCGTGGTGGTTTGGGGCGCTGGGCGTGTTGGGGTTGATTGTTGG
CAGCTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCGTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAACAGGCTATT
GCGGTCAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAATCGGTCTCTGC
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
TGGGGGCGTTGATCCTGTTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTTTCATTGAACGATGCCGTAGTCGGCGC
GATGGCGGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCCACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTTTGGTGGCCGCGTTGAGC
GGTCTGGTCGTTACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGTTAGCTTTTGGCCCCTGGCTGGC
AATGGGTGGCGTGTTTGGTGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

3D structure

• AlphaFold DB: A0A855MBX9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.37	96.085	0.484
pilD	Vibrio campbellii strain DS40M4	46.494	96.441	0.448
pilD	Neisseria gonorrhoeae MS11	48.627	90.747	0.441
pilD	Acinetobacter baumannii D1279779	43.59	97.153	0.423
pilD	Acinetobacter nosocomialis M2	43.223	97.153	0.42