Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MY9_RS05940 Genome accession   NC_017743
Coordinates   1188496..1192200 (+) Length   1234 a.a.
NCBI ID   WP_014663459.1    Uniprot ID   -
Organism   Bacillus sp. JS     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1183496..1197200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY9_RS05925 (MY9_1158) - 1183940..1184269 (-) 330 WP_014663456.1 four-helix bundle copper-binding protein -
  MY9_RS05930 (MY9_1159) - 1184388..1184825 (-) 438 WP_014663457.1 ferritin family protein -
  MY9_RS05935 (MY9_1160) addB 1185009..1188509 (+) 3501 WP_014663458.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MY9_RS05940 (MY9_1161) addA 1188496..1192200 (+) 3705 WP_014663459.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MY9_RS05945 (MY9_1162) sbcD 1192272..1193447 (+) 1176 WP_014663460.1 exonuclease subunit SbcD -
  MY9_RS05950 (MY9_1163) sbcC 1193444..1196836 (+) 3393 WP_014663461.1 exonuclease subunit SbcC -
  MY9_RS05955 (MY9_1164) hlpB 1196850..1197152 (+) 303 WP_014663462.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141317.61 Da        Isoelectric Point: 5.0619

>NTDB_id=50590 MY9_RS05940 WP_014663459.1 1188496..1192200(+) (addA) [Bacillus sp. JS]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEDLPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYRRVPAVAFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLTEMKPVIETLVQIVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAQNDKGEYEPSEAARFYQEQ
FYEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGKGTGRKIDLNKNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETDTELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRVFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGSALARHRDLEALAGNGTPVHADISGHPARFAVQMIHSYD
LLDDDVEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLNWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRALVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVQRLYEKELLTEEQKDAIDIEEIVQFFHTEIGG
QLIGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYD
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=50590 MY9_RS05940 WP_014663459.1 1188496..1192200(+) (addA) [Bacillus sp. JS]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTTGTTGAACGAATGATTCGGAAAATCACCGCAGAGGAAAACC
CAATTGATGTAGACCGCCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGAATTGCGGATGCC
TTGGAAAAAGAGCTTGTACAACGCCCCGGCTCGCTGCATATCAGACGGCAGCTTTCTCTTTTGAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTTGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTTAAACAAGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGACCTGCCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTAGCGCAGATTGATGAACT
GATTCAGCACCAGGACGATTTTAGTGAACTATATCGTCGTGTGCCAGCCGTCGCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCGGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCACTTGAAAAGCCTAACCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GATTGTTATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTCAGAATGACAAAGGTGAATATGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAACAG
TTTTATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTACAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGCGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGGAAAGGCACGGGGCGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGTTCAGATATATTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGACGAGACGGATACGGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACCGTGCAGTTTGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGAGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCAGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCTTACTATGAAGCGATGAAAGACTACTTGGCTGCTGGTGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGCGTCTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGACTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGCCAATATGAATCAACGGCATTTCGCGGCTTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAAGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGCCGGAATTTTAACATGATGGATTTAAATAAATCGTACCTGCTGGACAAGGA
GCTCGGATTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGAATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTCCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACATATTTAGATTTCATCGGATCGGCTCTTGCCAGACATAGAGACTTGGAGGCTTTGG
CTGGAAACGGTACACCAGTACACGCTGATATCTCAGGCCATCCAGCTCGTTTTGCCGTTCAAATGATTCATTCCTATGAT
TTGCTTGATGATGATGTGGAAGAAAGAATGGAAGAAAAAAGTGAACGTCTGGAAGCGATCCGCCGAGGGGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCAAGAGGTGACACAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCTTGTAAAACCGGCT
GACGGAAGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGTACTGCCATGCA
TACGGTGATGCAGCATATCCCGTTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCAAAGGCTTTATGAAA
AAGAGCTGCTTACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAGATCGGGGGA
CAGCTGATCGGTGCGAAATGGAAGGACCGGGAAATACCTTTCAGCTTGGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACACGAGGCGGATGAGCCGCTTTTAGTGCAGGGGATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATTTATTGG
ATTATAAGTCGGACCGGATTGAGGGCAAATTCCAACATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAT
ACCCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTCACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.326

100

0.973

Multiple sequence alignment