Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MY9_RS05935 Genome accession   NC_017743
Coordinates   1185009..1188509 (+) Length   1166 a.a.
NCBI ID   WP_014663458.1    Uniprot ID   -
Organism   Bacillus sp. JS     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1180009..1193509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY9_RS05915 (MY9_1156) - 1180861..1182063 (-) 1203 WP_014663454.1 MFS transporter -
  MY9_RS05920 (MY9_1157) - 1182177..1183904 (+) 1728 WP_014663455.1 SgrR family transcriptional regulator -
  MY9_RS05925 (MY9_1158) - 1183940..1184269 (-) 330 WP_014663456.1 four-helix bundle copper-binding protein -
  MY9_RS05930 (MY9_1159) - 1184388..1184825 (-) 438 WP_014663457.1 ferritin family protein -
  MY9_RS05935 (MY9_1160) addB 1185009..1188509 (+) 3501 WP_014663458.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MY9_RS05940 (MY9_1161) addA 1188496..1192200 (+) 3705 WP_014663459.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MY9_RS05945 (MY9_1162) sbcD 1192272..1193447 (+) 1176 WP_014663460.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134582.47 Da        Isoelectric Point: 5.4728

>NTDB_id=50589 MY9_RS05935 WP_014663458.1 1185009..1188509(+) (addB) [Bacillus sp. JS]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKNGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEARPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLSEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETEVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVVKRLEELFPHHKERLLTNEPEQVSDEEQLKYVVNQS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRTLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=50589 MY9_RS05935 WP_014663458.1 1185009..1188509(+) (addB) [Bacillus sp. JS]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTACG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACAGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAACGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGGTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAATATCGCGGAGAACGTGTGTTATCTGAA
AAGCTCCATGACTTATCGATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATGTAAAAGGCGCGCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGCTGGAGCAGCTTATGGTTCATGCAGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTAGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACACAGACACCTGAATTGGCCCATCTAG
AGGCGCAGTATGAAGCGCGTCCTGCCGTTCCTTACGCAGAAAAACAAGAAGCACTGACTGTAATGCAGGCCGCGAACAGA
CGAGCTGAGCTGGAAGGTATTGCCCGGGAAATTCACGCCCTCGTCAGAGAGAAAGGATATCGATATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGCTATTCCCGCTCAGTGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCTTATGGGATCAAAGGCGACCGCTGGACAAAGGGCGACCGTTTCCAATATAGGCGCT
TTGTGTCGCTGGATGATGATTTTGCGCAGACCGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTACAGGAGAAGGCAGAGGCGCTCTATCG
CTATTTAGAAGAGACGGAGGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCGCTTATTCCGCCTGCGCTTGACCA
GGTATTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACATCCTGTACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTTCTGTCGGATGATGACCGGGAATGGCTGAAAACGATCGGGGTTGAGCTTTCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAGCATTTCCTCATCTACATGGCATTTTCAAGTCCGTCTGACCGGCTTTATGT
ATCGTATCCGATTGCAGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCGTTAAACGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGAAGTATGTTGTCAATCAAAGC
GTGGCACAGTCCTTTACTGCGAGTCAGCTCAGATTATGGACCCGGGAATATGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTAAAGC
AGCTTGAACGCAGTGTGTCAAGACAGCTCTATGGTGAACGCATTCAGGGCAGCGTATCGAGAATGGAAACCTTTAACGCA
TGTCCGTTTTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTTGAAGCGCCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGAGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACGCGTGTGTCCGGTATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTCGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGTATTGACCGTGTGGATAAAGCTGAAAGCTCAAAAGGCCTGCTCCTCAGAATTGTC
GATTATAAATCAAGCGATAAAGGGCTTGACTTAGCGGAAGTATATTACGGATTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCCGACTGGCTCGGAATGAGGGCAACGCCTGCAGGAGTGCTGTATTTCCATATTCATGATC
CGATGATTCAATCTAATCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATTTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGTGGTTCGCCTTATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGTCT
GAAAAAAGACGGCTCTCTCAGATCAGACTCGGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GTACCTTCCAAGAAGCAGGCGAACAAATTACCGACGGCCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTGTGCCAATTTGATGAATCACTAGAGGAAAACGAGTATCGCACATTAAAAGCTGA
AAAAGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(306-668)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.885

100

0.989

Multiple sequence alignment