Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IWH25_RS02800 Genome accession   NZ_CP064781
Coordinates   588783..590219 (+) Length   478 a.a.
NCBI ID   WP_203387841.1    Uniprot ID   A0A974SPZ0
Organism   Azospira restricta strain DSM 18626     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583783..595219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS02765 (IWH25_02765) nusB 583949..584452 (+) 504 WP_203387834.1 transcription antitermination factor NusB -
  IWH25_RS02770 (IWH25_02770) thiL 584465..585427 (+) 963 WP_203387835.1 thiamine-phosphate kinase -
  IWH25_RS02775 (IWH25_02775) - 585420..585911 (+) 492 WP_238998979.1 phosphatidylglycerophosphatase A -
  IWH25_RS02780 (IWH25_02780) pncC 585911..586408 (+) 498 WP_203387837.1 nicotinamide-nucleotide amidase -
  IWH25_RS02785 (IWH25_02785) recA 586528..587550 (+) 1023 WP_203387838.1 recombinase RecA Machinery gene
  IWH25_RS02790 (IWH25_02790) recX 587554..588000 (+) 447 WP_203387839.1 recombination regulator RecX -
  IWH25_RS02795 (IWH25_02795) lptB 588003..588740 (+) 738 WP_203387840.1 LPS export ABC transporter ATP-binding protein -
  IWH25_RS02800 (IWH25_02800) rpoN/rpoN1 588783..590219 (+) 1437 WP_203387841.1 RNA polymerase factor sigma-54 Machinery gene
  IWH25_RS02805 (IWH25_02805) hpf 590233..590559 (+) 327 WP_203387842.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  IWH25_RS02810 (IWH25_02810) ptsN 590655..591101 (+) 447 WP_203387843.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IWH25_RS02815 (IWH25_02815) hprK 591112..592047 (+) 936 WP_203387844.1 HPr(Ser) kinase/phosphatase -
  IWH25_RS02820 (IWH25_02820) - 592100..592417 (+) 318 WP_203387845.1 PsiF family protein -
  IWH25_RS02825 (IWH25_02825) - 592516..593187 (+) 672 WP_203387846.1 hypothetical protein -
  IWH25_RS02830 (IWH25_02830) - 593207..593998 (+) 792 WP_203387847.1 hypothetical protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 52757.83 Da        Isoelectric Point: 4.9412

>NTDB_id=504693 IWH25_RS02800 WP_203387841.1 588783..590219(+) (rpoN/rpoN1) [Azospira restricta strain DSM 18626]
MRPALQLKLSQHLALTPQLQQSIRLLQLSTLELNQELEKFLLENPLLEREDEDNFVPAPSSSSTSEREPAAEAGGEGEAG
SSDEVRPNTDDEGWPGDGSPGTGSSGSGGSDEDGDYQDIQAATTSLSDHLIWQLGLTPLPDRDRMLVRVLIEALDEDGYL
SQTLDELAEIMPPELEIEPEELQIALKHLQHFDPTGVGARNAQECLSLQLTALPADPVRDLALVIVRQHLELLAARDFAR
IKKLAGCDDDLLRAAHALIKGLNPRPGAQFAPLDARYIIPDVIVKKVRNQWRASINPDAYPRLRINRLYAEVLAGQRGSG
LSGQLQEARWLIKNVQQRFDTIQRVSQTIVDRQRQFFEHGEVAMRPLVLREIADILGLHESTVSRVTTQKYMATPRGIFE
LKYFFGSHVATDTGGACSATAIRALIKQLVGAEDAKKPLSDSQIAEILGQQGIVVARRTIAKYREALNIPPVNLRKSL

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=504693 IWH25_RS02800 WP_203387841.1 588783..590219(+) (rpoN/rpoN1) [Azospira restricta strain DSM 18626]
ATGAGACCCGCTCTCCAGCTCAAGCTCTCGCAGCACCTTGCGTTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTTGAGCTCAATCAGGAGCTGGAAAAGTTCCTGCTCGAGAACCCGCTGCTCGAGCGAGAGGATGAGGACA
ATTTCGTCCCGGCACCGTCGTCGAGCAGCACCAGCGAACGCGAGCCGGCCGCTGAGGCCGGCGGCGAAGGCGAGGCCGGC
AGCAGCGACGAGGTTCGCCCGAACACCGACGACGAAGGCTGGCCCGGCGACGGCAGTCCGGGAACCGGCTCGTCCGGCAG
CGGCGGCAGCGACGAGGACGGCGATTACCAGGACATCCAGGCGGCGACCACCTCGCTCTCCGACCACCTGATCTGGCAGC
TCGGCCTGACGCCGCTGCCCGACCGCGACCGCATGCTGGTGCGCGTGCTGATCGAGGCCCTCGACGAGGACGGCTACCTG
TCGCAGACGCTCGACGAACTGGCCGAGATCATGCCGCCCGAACTGGAGATCGAGCCCGAGGAACTGCAGATCGCGCTGAA
GCACCTGCAGCACTTCGACCCGACCGGCGTCGGCGCGCGCAACGCGCAGGAATGCCTGTCGCTGCAGCTGACGGCGCTCC
CCGCCGATCCCGTGCGCGACCTGGCGCTGGTCATCGTCCGCCAGCATCTGGAATTGCTCGCCGCCCGCGACTTCGCCCGC
ATCAAGAAGCTGGCCGGCTGCGACGACGACCTGCTGCGCGCCGCGCACGCACTGATCAAGGGCCTCAACCCGCGCCCCGG
CGCCCAGTTCGCGCCGCTCGACGCACGCTACATCATCCCCGACGTGATCGTGAAGAAGGTGCGCAACCAGTGGCGGGCGA
GCATCAACCCCGACGCCTACCCGCGCCTGCGCATCAACCGCCTCTACGCCGAGGTGCTCGCCGGCCAGCGCGGCTCGGGA
CTCTCCGGCCAGCTGCAGGAAGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGCTTCGACACCATCCAGCGCGTGTCGCA
GACGATCGTTGACCGCCAGCGCCAGTTCTTCGAACATGGCGAGGTAGCGATGCGGCCGCTGGTGCTGCGCGAGATTGCCG
ACATCCTCGGCCTGCACGAATCGACCGTATCGCGGGTAACGACGCAGAAATACATGGCCACCCCGCGCGGCATCTTCGAA
CTGAAATACTTCTTCGGCAGCCATGTCGCCACCGATACCGGCGGCGCCTGCTCGGCCACCGCGATCCGTGCGCTGATCAA
GCAGCTGGTGGGTGCCGAAGATGCGAAAAAACCGCTATCTGACAGCCAGATAGCGGAAATCCTTGGCCAGCAGGGGATTG
TCGTGGCGCGACGAACGATTGCAAAATATCGTGAAGCGCTGAACATCCCGCCGGTCAATCTACGCAAGAGCCTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(322-476)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.738

100

0.609