Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   HCD_RS00200 Genome accession   NC_017735
Coordinates   39219..41594 (-) Length   791 a.a.
NCBI ID   WP_014658597.1    Uniprot ID   I0EQ49
Organism   Helicobacter cetorum MIT 99-5656     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34219..46594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCD_RS00180 (HCD_00160) lpxE 34598..35182 (+) 585 WP_014658593.1 lipid A 1-phosphatase LpxE -
  HCD_RS00185 (HCD_00165) nspC 35179..36402 (-) 1224 WP_014658594.1 carboxynorspermidine decarboxylase -
  HCD_RS00190 (HCD_00170) - 36399..37364 (-) 966 WP_014658595.1 chemotaxis protein -
  HCD_RS00195 (HCD_00175) - 37435..38820 (-) 1386 WP_041594796.1 COG3014 family protein -
  HCD_RS00200 (HCD_00180) comB4 39219..41594 (-) 2376 WP_014658597.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  HCD_RS00205 (HCD_00185) comB3 41596..41859 (-) 264 WP_014658598.1 hypothetical protein Machinery gene
  HCD_RS00210 (HCD_00190) comB2 41859..42140 (-) 282 WP_014658599.1 TrbC/VirB2 family protein Machinery gene
  HCD_RS00215 (HCD_00195) - 42291..43118 (-) 828 WP_014658600.1 DUF5718 family protein -
  HCD_RS00220 (HCD_00200) - 43212..44264 (-) 1053 WP_014658601.1 MnmA/TRMU family protein -
  HCD_RS00225 (HCD_00205) dnaG 44261..45967 (-) 1707 WP_014658602.1 DNA primase -

Sequence

Protein


Download         Length: 791 a.a.        Molecular weight: 90743.51 Da        Isoelectric Point: 9.1112

>NTDB_id=50445 HCD_RS00200 WP_014658597.1 39219..41594(-) (comB4) [Helicobacter cetorum MIT 99-5656]
MLEKLVSTIKQKVSNYLLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILSLEGISYTHLNMEQLQDLFTERQMALD
SLEKVVVRIVAKRRKMDYRQNTQAKSKFVQAILDKFENKEVYENQYFLVLESTHSLWGVLEHKKQSLMRANKENFKDILS
YKSHFLQETLKSLELQLKSYRPKLLNSREVLNFYAEYINGFELPLKPLVGGYLSDSYIASLVTFEKDYFIQERFNQKLYS
RLIGIKAYESERITSIAIATLLYQEAHLDIVFSIEPMSVNKALNFLKERTKFSMSNLVKNELLEYQELVKTRRLSMQKFA
LNILIKAPSLESLDAQTSLVLGLLSKEGLVGVVETFGLKGGYFSFFPECIHLNHRLRFLTSKALACLMVFEKQNSGFRAN
SWGNSPLSVFKNLDSTPFLFNFHNQEVKHDRGSEVAKVNGHTLIIGATGSGKSTLMSFLMMSALKYEKMRLLAFDRMQGL
YSFTKFFKGHYHDGLSFSINPFSLEPNAQNMQFLQSFYQAMLNIKDPTSRNKELIEDVNAISNAIMSLYETLYPKAFDLK
DFKETLKRTSSNQLNLSLEPYLSNPLFNALNDTLNTNALLNVINLDAITQNPKDLGLLAYYLFYRILEESRKNDGGFLVF
LDEFKSYVENDLLNTKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNAKELKQNFNVPLSEME
ISFLENTPLHSRQVLVKNLGDGSSNMIDVSLESLGHYLKIFNSDSSHVNKMKALQQDYPNKWREKLIGESN

Nucleotide


Download         Length: 2376 bp        

>NTDB_id=50445 HCD_RS00200 WP_014658597.1 39219..41594(-) (comB4) [Helicobacter cetorum MIT 99-5656]
ATGTTAGAAAAGCTTGTAAGCACTATCAAGCAAAAGGTTTCAAACTATCTTTTAGGGGTTTTGCCTAAAAGCTATTCAAT
GAGTGAAGAAAACAATATTCTAGGCTTGTATGATGAGCATTTTTTACTCACTAAGAATGAAAATTTGGTCGGCATACTCA
GTTTGGAAGGGATTAGCTACACGCATTTGAATATGGAGCAACTACAAGATTTATTCACTGAACGCCAAATGGCGTTAGAT
TCCTTAGAAAAAGTCGTGGTGCGCATTGTAGCTAAAAGGCGTAAGATGGACTACAGACAGAACACTCAAGCAAAATCTAA
GTTCGTGCAAGCTATTTTGGATAAATTTGAAAATAAAGAAGTGTATGAAAATCAATATTTCTTAGTCTTAGAAAGCACGC
ATTCTTTATGGGGCGTTTTAGAGCATAAGAAACAATCTCTTATGCGTGCTAATAAAGAAAATTTTAAGGATATTCTCTCT
TATAAATCACATTTTTTACAAGAAACTTTAAAAAGCCTGGAACTGCAACTCAAAAGTTATCGCCCCAAACTCTTAAACTC
TAGAGAAGTTTTGAACTTTTATGCCGAATATATCAATGGGTTTGAACTGCCCCTAAAGCCCCTAGTAGGGGGGTATTTGA
GCGATAGCTATATCGCTAGTTTGGTAACTTTTGAAAAAGATTATTTCATTCAAGAGCGTTTTAATCAAAAACTCTATAGT
CGCTTAATCGGTATCAAAGCCTATGAGAGTGAACGCATCACTTCTATAGCCATTGCCACGCTTTTATACCAAGAAGCTCA
TTTGGACATTGTGTTTTCTATAGAGCCTATGAGCGTGAATAAGGCGTTGAATTTTTTAAAAGAACGCACCAAGTTTAGCA
TGTCTAATCTAGTCAAAAATGAGTTGTTAGAATATCAAGAGTTGGTTAAAACAAGGCGTTTGTCTATGCAAAAATTCGCC
TTAAACATTCTTATAAAAGCCCCTAGTTTAGAGAGTTTAGACGCTCAAACAAGCTTAGTTTTAGGGCTTTTATCTAAAGA
AGGTCTAGTGGGCGTAGTAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCTTTTTTTCCAGAATGCATTCATTTGAACC
ACCGCTTGCGTTTTTTGACTTCTAAAGCTTTGGCATGTTTAATGGTATTTGAAAAACAAAATTCAGGTTTTAGGGCTAAT
TCATGGGGGAATAGTCCCTTAAGTGTGTTTAAAAATTTGGACTCAACCCCTTTTTTATTTAACTTTCATAACCAAGAAGT
TAAGCATGATAGGGGTAGCGAAGTTGCTAAAGTCAATGGACACACGCTTATTATTGGGGCTACAGGAAGCGGTAAAAGCA
CTTTAATGAGTTTTTTAATGATGAGTGCTTTGAAGTATGAAAAAATGCGCCTTTTAGCCTTTGACAGAATGCAAGGCTTA
TATTCTTTCACAAAGTTTTTTAAGGGGCATTATCATGATGGGCTTAGTTTTAGCATTAACCCTTTTTCTTTAGAGCCAAA
CGCACAGAACATGCAATTTTTGCAATCCTTTTATCAAGCGATGTTAAACATAAAAGACCCCACTTCACGCAATAAAGAAT
TGATAGAAGATGTGAATGCGATTTCTAATGCCATTATGAGTCTTTATGAAACCTTGTATCCTAAAGCCTTTGATTTAAAG
GATTTTAAAGAAACGCTTAAAAGAACTTCATCTAATCAATTAAATTTGAGTTTAGAGCCGTATTTGAGTAACCCCCTTTT
TAACGCTCTTAATGATACATTAAATACAAACGCCTTGTTAAATGTTATTAATTTAGACGCTATCACTCAAAACCCTAAAG
ATTTAGGGCTTTTAGCCTATTATTTGTTTTATAGAATTTTAGAAGAGTCTAGGAAAAATGATGGTGGGTTTTTGGTTTTT
TTAGATGAGTTCAAATCTTATGTAGAAAATGATTTGCTTAATACCAAGATTAACGCTTTAATCACGCAAGCACGAAAGGC
TAATGGCGTGGTGGTGTTAGCCTTACAGGATATTTACCAACTAAGTGGGGTAAAAAACGCTCATAGTTTTTTAAGCAACA
TGGGAACGCTTATTTTATACCCCCAAAAAAACGCCAAAGAGTTAAAACAAAATTTTAATGTGCCTTTAAGTGAAATGGAA
ATTTCTTTTTTAGAAAACACTCCTTTGCATTCAAGACAGGTTCTAGTCAAAAATCTAGGCGATGGGAGTTCTAATATGAT
TGATGTGAGTTTAGAGAGTTTAGGGCATTATCTAAAAATTTTTAACTCTGATTCTAGCCATGTTAATAAGATGAAAGCTT
TACAACAAGATTATCCTAACAAGTGGCGTGAAAAATTGATAGGAGAATCAAACTAG

Domains

Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(401-466)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0EQ49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

85.769

99.494

0.853

Multiple sequence alignment