Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   KQ217_RS14225 Genome accession   NZ_CP076706
Coordinates   2922590..2924788 (-) Length   732 a.a.
NCBI ID   WP_216803434.1    Uniprot ID   -
Organism   Escherichia coli O170:H18 strain CFIAFB20120266     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2917590..2929788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ217_RS14205 (KQ217_14205) hslU 2918394..2919725 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  KQ217_RS14210 (KQ217_14210) hslV 2919735..2920265 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  KQ217_RS14215 (KQ217_14215) ftsN 2920358..2921317 (-) 960 WP_000068834.1 cell division protein FtsN -
  KQ217_RS14220 (KQ217_14220) cytR 2921409..2922434 (-) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  KQ217_RS14225 (KQ217_14225) priA 2922590..2924788 (-) 2199 WP_216803434.1 primosomal protein N' Machinery gene
  KQ217_RS14230 (KQ217_14230) rpmE 2924991..2925203 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  KQ217_RS14235 (KQ217_14235) yiiX 2925264..2925872 (-) 609 WP_088170576.1 YiiX family permuted papain-like enzyme -
  KQ217_RS14240 (KQ217_14240) metJ 2925932..2926249 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  KQ217_RS14245 (KQ217_14245) metB 2926526..2927686 (+) 1161 WP_000197205.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81783.13 Da        Isoelectric Point: 9.0293

>NTDB_id=504247 KQ217_RS14225 WP_216803434.1 2922590..2924788(-) (priA) [Escherichia coli O170:H18 strain CFIAFB20120266]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQRKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=504247 KQ217_RS14225 WP_216803434.1 2922590..2924788(-) (priA) [Escherichia coli O170:H18 strain CFIAFB20120266]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTCCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGAGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTATCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCAGCGTTACGGCAACGCAAAATCTGGCGTGATCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGGTTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCGGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGTGGTTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCACTGGCTGAACGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTCATTCTCGCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCCCCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGCTGGATGTCGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.047

100

0.454