Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KQ221_RS18740 Genome accession   NZ_CP076704
Coordinates   3875343..3875783 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli O8:H28 strain BMH-17-0027     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3870343..3880783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ221_RS18720 (KQ221_18720) aroP 3871341..3872714 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  KQ221_RS18725 (KQ221_18725) ampE 3872757..3873611 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KQ221_RS18730 (KQ221_18730) ampD 3873608..3874159 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KQ221_RS18735 (KQ221_18735) nadC 3874247..3875140 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KQ221_RS18740 (KQ221_18740) pilA 3875343..3875783 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  KQ221_RS18745 (KQ221_18745) pilB 3875793..3877178 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  KQ221_RS18750 (KQ221_18750) hofC 3877168..3878370 (+) 1203 WP_000157234.1 protein transport protein HofC -
  KQ221_RS18755 (KQ221_18755) guaC 3878405..3879448 (-) 1044 WP_001217338.1 GMP reductase -
  KQ221_RS18760 (KQ221_18760) - 3879604..3879648 (-) 45 WP_120795372.1 protein YacM -
  KQ221_RS18765 (KQ221_18765) coaE 3879673..3880293 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=504170 KQ221_RS18740 WP_000360894.1 3875343..3875783(+) (pilA) [Escherichia coli O8:H28 strain BMH-17-0027]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=504170 KQ221_RS18740 WP_000360894.1 3875343..3875783(+) (pilA) [Escherichia coli O8:H28 strain BMH-17-0027]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377