Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI17_RS00310 Genome accession   NZ_CP064622
Coordinates   65676..67025 (-) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG344     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 37765..72196 65676..67025 within 0


Gene organization within MGE regions


Location: 37765..72196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI17_RS00175 (HWI17_00180) ccrB 37765..39393 (-) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  HWI17_RS00180 (HWI17_00185) ccrA 39390..40751 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  HWI17_RS00185 (HWI17_00190) - 40938..41567 (-) 630 Protein_34 hypothetical protein -
  HWI17_RS00190 (HWI17_00195) - 41576..41914 (+) 339 Protein_35 recombinase family protein -
  HWI17_RS00195 (HWI17_00200) - 42026..42385 (-) 360 WP_001631039.1 hypothetical protein -
  HWI17_RS00200 (HWI17_00205) - 42592..42918 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI17_RS00205 (HWI17_00210) - 43248..45173 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  HWI17_RS00210 (HWI17_00215) - 46296..47246 (-) 951 Protein_39 transposase -
  HWI17_RS00215 (HWI17_00220) - 47367..47936 (+) 570 WP_002470562.1 hypothetical protein -
  HWI17_RS00220 (HWI17_00225) - 47981..49138 (+) 1158 WP_002485721.1 hypothetical protein -
  HWI17_RS00225 (HWI17_00230) - 49321..49593 (+) 273 WP_002501902.1 hypothetical protein -
  HWI17_RS00230 (HWI17_00235) - 49623..50297 (+) 675 WP_002501901.1 IS6-like element IS257 family transposase -
  HWI17_RS00235 (HWI17_00240) lspA 50325..50777 (-) 453 WP_164071529.1 signal peptidase II -
  HWI17_RS00240 (HWI17_00245) - 50935..51336 (+) 402 WP_057504659.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI17_RS00245 (HWI17_00250) - 51590..52357 (+) 768 WP_057504658.1 ZIP family metal transporter -
  HWI17_RS00250 (HWI17_00255) - 52614..55022 (+) 2409 WP_107362604.1 heavy metal translocating P-type ATPase -
  HWI17_RS00255 (HWI17_00260) - 55238..55912 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI17_RS00260 (HWI17_00265) - 55944..56690 (+) 747 WP_408023211.1 glycerophosphoryl diester phosphodiesterase -
  HWI17_RS00265 (HWI17_00270) - 56787..57215 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI17_RS00270 (HWI17_00275) mecA 57261..59270 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI17_RS00275 (HWI17_00280) - 59367..60353 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI17_RS00280 (HWI17_00285) - 60343..60585 (+) 243 Protein_53 type I restriction enzyme endonuclease domain-containing protein -
  HWI17_RS00285 (HWI17_00290) - 60576..62186 (-) 1611 Protein_54 IS1182 family transposase -
  HWI17_RS00290 (HWI17_00295) - 62235..62741 (-) 507 WP_001092194.1 DUF1643 domain-containing protein -
  HWI17_RS00295 (HWI17_00300) - 62756..63067 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI17_RS00300 (HWI17_00305) - 63154..63504 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI17_RS00305 (HWI17_00310) ccrB 64026..65654 (-) 1629 WP_408023194.1 cassette chromosome recombinase CcrB Machinery gene
  HWI17_RS00310 (HWI17_00315) ccrA 65676..67025 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  HWI17_RS00315 (HWI17_00320) cch1 67259..69052 (-) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  HWI17_RS00320 (HWI17_00325) ssb 69052..69348 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  HWI17_RS00325 (HWI17_00330) - 69541..70587 (-) 1047 WP_001042023.1 hypothetical protein -
  HWI17_RS00330 (HWI17_00335) - 71042..72196 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=503670 HWI17_RS00310 WP_000815640.1 65676..67025(-) (ccrA) [Staphylococcus epidermidis strain UMCG344]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=503670 HWI17_RS00310 WP_000815640.1 65676..67025(-) (ccrA) [Staphylococcus epidermidis strain UMCG344]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759