Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KO461_RS04285 Genome accession   NZ_CP076449
Coordinates   825468..826157 (-) Length   229 a.a.
NCBI ID   WP_005594882.1    Uniprot ID   Q8KRY3
Organism   Aggregatibacter actinomycetemcomitans strain CU1000N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 820468..831157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO461_RS04260 (KO461_04275) - 820636..821832 (-) 1197 WP_005564236.1 WD40 repeat domain-containing protein -
  KO461_RS04265 (KO461_04280) - 821835..823943 (-) 2109 WP_094978696.1 alpha/beta hydrolase -
  KO461_RS04270 - 823966..824142 (-) 177 WP_228414662.1 hypothetical protein -
  KO461_RS04275 - 824151..824729 (-) 579 WP_005566183.1 DUF4123 domain-containing protein -
  KO461_RS04280 - 824733..825299 (-) 567 WP_228414663.1 hypothetical protein -
  KO461_RS04285 (KO461_04295) pilD 825468..826157 (-) 690 WP_005594882.1 prepilin peptidase Machinery gene
  KO461_RS04290 (KO461_04300) pilC 826154..827377 (-) 1224 WP_005540455.1 type II secretion system F family protein Machinery gene
  KO461_RS04295 (KO461_04305) - 827370..828779 (-) 1410 Protein_845 GspE/PulE family protein -
  KO461_RS04300 (KO461_04310) pilA 828806..829258 (-) 453 WP_005550263.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KO461_RS04305 (KO461_04315) ampD 829380..829934 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KO461_RS04310 (KO461_04320) nanQ 830408..830878 (-) 471 WP_005540462.1 N-acetylneuraminate anomerase -

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26056.79 Da        Isoelectric Point: 7.9832

>NTDB_id=502607 KO461_RS04285 WP_005594882.1 825468..826157(-) (pilD) [Aggregatibacter actinomycetemcomitans strain CU1000N]
MMWLLFLLGGVLCGWRVYHYVEHFADNLSREVHAAYTEIYPQNPPHFTAENSVLKPLKRHFSAHLYSLLFTLLFMICYAI
NEPLQALCFALYGALLIVISLVDWHYRLISPALCQALFALGLGAAYWQLGTLTLAQSLQSAVIFFGVFYAIYHAAKWYYR
QEALGRGDYWLALGLGVFLPAQQLPVFLFLACFFGLLYAAYAKYQHRTLNTMPFGPFMSLAGVVCLLLN

Nucleotide


Download         Length: 690 bp        

>NTDB_id=502607 KO461_RS04285 WP_005594882.1 825468..826157(-) (pilD) [Aggregatibacter actinomycetemcomitans strain CU1000N]
ATGATGTGGCTACTTTTTCTGCTTGGCGGTGTGCTTTGTGGCTGGCGCGTTTATCATTACGTAGAACATTTCGCCGATAA
CCTCAGCCGCGAGGTTCATGCCGCCTACACGGAAATTTACCCGCAAAACCCACCGCACTTTACGGCGGAAAACTCGGTTC
TAAAACCGCTTAAACGGCATTTTTCCGCACATCTTTACAGCCTTTTATTCACCTTGCTGTTCATGATTTGTTATGCCATC
AATGAACCGTTACAAGCCCTTTGTTTTGCACTGTATGGCGCCTTATTAATCGTCATTTCTTTGGTGGATTGGCACTATCG
CCTGATTTCACCCGCCTTATGCCAAGCCTTGTTCGCTCTCGGTTTAGGCGCGGCATACTGGCAACTCGGCACATTAACAT
TGGCGCAAAGCCTGCAAAGTGCGGTCATTTTTTTCGGTGTTTTTTATGCCATCTACCACGCCGCAAAATGGTACTACCGA
CAAGAAGCTCTCGGACGCGGCGATTATTGGCTGGCACTGGGCTTAGGCGTTTTTCTACCGGCACAACAACTTCCCGTTTT
TTTATTCCTCGCCTGCTTTTTCGGCTTACTTTATGCCGCCTACGCAAAATACCAACACCGCACACTCAACACCATGCCTT
TCGGTCCCTTTATGTCCCTAGCCGGCGTGGTCTGTTTATTGCTTAATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(94-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KRY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

38.865

100

0.389
  pilD Haemophilus influenzae Rd KW20

38.393

97.817

0.376