Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   HWI55_RS00480 Genome accession   NZ_CP064485
Coordinates   103133..104479 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus epidermidis strain UMCG382     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 64187..104479 103133..104479 within 0


Gene organization within MGE regions


Location: 64187..104479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI55_RS00290 (HWI55_00280) ccrB 64187..65815 (-) 1629 WP_407567791.1 cassette chromosome recombinase CcrB Machinery gene
  HWI55_RS00295 (HWI55_00285) ccrA 65812..67173 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  HWI55_RS00300 (HWI55_00290) - 67360..67989 (-) 630 Protein_57 hypothetical protein -
  HWI55_RS00305 (HWI55_00295) - 67998..68336 (+) 339 Protein_58 recombinase family protein -
  HWI55_RS00310 (HWI55_00300) - 68448..68807 (-) 360 WP_001631039.1 hypothetical protein -
  HWI55_RS00315 (HWI55_00305) - 69014..69340 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI55_RS00320 (HWI55_00310) - 69670..71595 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  HWI55_RS00325 (HWI55_00315) - 72821..74077 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  HWI55_RS00330 (HWI55_00320) - 74492..74731 (+) 240 WP_001282728.1 hypothetical protein -
  HWI55_RS00335 (HWI55_00325) - 74763..75437 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI55_RS00340 (HWI55_00330) - 75528..76511 (-) 984 Protein_65 protein rep -
  HWI55_RS00345 (HWI55_00335) mobV 76756..78018 (-) 1263 WP_000119405.1 MobV family relaxase -
  HWI55_RS00350 (HWI55_00340) bleO 78528..78932 (-) 405 WP_001242578.1 bleomycin binding protein -
  HWI55_RS00355 (HWI55_00345) aadD1 79149..79919 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  HWI55_RS00360 (HWI55_00350) - 80112..80786 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  HWI55_RS00365 (HWI55_00355) - 80822..81211 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  HWI55_RS00370 (HWI55_00360) - 82008..82751 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  HWI55_RS00375 (HWI55_00365) - 82848..83276 (+) 429 WP_000872606.1 MaoC family dehydratase -
  HWI55_RS00380 (HWI55_00370) mecA 83322..85331 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI55_RS00385 (HWI55_00375) mecR1 85427..87184 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  HWI55_RS00390 (HWI55_00380) mecI 87184..87555 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  HWI55_RS00395 (HWI55_00385) psm-mec 87640..87708 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  HWI55_RS00400 (HWI55_00390) - 88042..89172 (+) 1131 WP_010922808.1 ROK family transcriptional regulator -
  HWI55_RS00405 (HWI55_00395) cstB 89286..90620 (-) 1335 Protein_78 persulfide dioxygenase-sulfurtransferase CstB -
  HWI55_RS00410 (HWI55_00400) - 90653..91717 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  HWI55_RS00415 (HWI55_00405) cstR 91853..92113 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  HWI55_RS00420 (HWI55_00410) - 92113..92751 (+) 639 Protein_81 sulfite exporter TauE/SafE family protein -
  HWI55_RS00425 (HWI55_00420) - 93025..93641 (-) 617 Protein_82 CadD family cadmium resistance transporter -
  HWI55_RS00430 (HWI55_00425) - 93722..96136 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  HWI55_RS00435 (HWI55_00430) - 96129..96494 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  HWI55_RS00440 (HWI55_00435) - 96732..97109 (-) 378 WP_001041908.1 DUF6262 family protein -
  HWI55_RS00445 (HWI55_00440) - 97116..99008 (-) 1893 WP_002485298.1 site-specific integrase -
  HWI55_RS00450 (HWI55_00445) - 99203..99526 (-) 324 WP_000088091.1 JAB domain-containing protein -
  HWI55_RS00455 (HWI55_00450) - 99519..99725 (-) 207 WP_001077283.1 hypothetical protein -
  HWI55_RS00460 (HWI55_00455) - 99727..100248 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  HWI55_RS00465 (HWI55_00460) - 100267..100578 (-) 312 WP_000833212.1 DUF960 family protein -
  HWI55_RS00470 (HWI55_00465) - 100663..101013 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI55_RS00475 (HWI55_00470) ccrB 101484..103112 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  HWI55_RS00480 (HWI55_00475) ccrA 103133..104479 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=502295 HWI55_RS00480 WP_000816499.1 103133..104479(-) (ccrA) [Staphylococcus epidermidis strain UMCG382]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=502295 HWI55_RS00480 WP_000816499.1 103133..104479(-) (ccrA) [Staphylococcus epidermidis strain UMCG382]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725