Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   IQ680_RS13370 Genome accession   NZ_CP064266
Coordinates   2595736..2599461 (+) Length   1241 a.a.
NCBI ID   WP_243521100.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain N451-2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2590736..2604461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ680_RS13355 - 2590832..2591425 (+) 594 WP_243521098.1 TVP38/TMEM64 family protein -
  IQ680_RS13360 lepB 2591483..2592046 (+) 564 WP_098337492.1 signal peptidase I -
  IQ680_RS13365 addB 2592224..2595739 (+) 3516 WP_243521099.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  IQ680_RS13370 addA 2595736..2599461 (+) 3726 WP_243521100.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  IQ680_RS13375 - 2599537..2599830 (+) 294 WP_098337489.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  IQ680_RS13380 gerPF 2599916..2600131 (-) 216 WP_098337488.1 spore germination protein GerPF -
  IQ680_RS13385 - 2600177..2600563 (-) 387 WP_243521101.1 spore germination protein GerPE -
  IQ680_RS13390 - 2600577..2600771 (-) 195 WP_001104655.1 hypothetical protein -
  IQ680_RS13395 - 2600778..2601392 (-) 615 WP_098337486.1 spore germination protein GerPC -
  IQ680_RS13400 - 2601473..2601664 (-) 192 WP_243521102.1 spore germination protein GerPB -
  IQ680_RS13405 - 2601681..2601902 (-) 222 WP_243521103.1 spore germination protein -
  IQ680_RS13410 - 2602025..2602204 (-) 180 WP_098337482.1 aspartyl-phosphate phosphatase Spo0E family protein -
  IQ680_RS13415 - 2602447..2603346 (+) 900 WP_243521104.1 fumarylacetoacetate hydrolase family protein -
  IQ680_RS13420 - 2603318..2603821 (+) 504 WP_243521105.1 HXXEE domain-containing protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 143331.64 Da        Isoelectric Point: 4.9245

>NTDB_id=500412 IQ680_RS13370 WP_243521100.1 2595736..2599461(+) (addA) [Bacillus pseudomycoides strain N451-2]
MIENWPQKPEGSQWTDDQWKAVVRDGRDILVAAAAGSGKTAVLVERIIRKIINAENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRTYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGTE
GNDIFFELVDRYTSDRSDDDLQRMILALHTESRAHPDPEKWLDKLVEAYDVEGKSIEDLTYASYLLEDVKFQLETAEKHV
QKAIELAMLPDGPAPRAETLQADIMLLGTLSSAAQQSWTSVYEAMQNVSWQTLKRIKKSDYNEEIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFKEMHPVLEKLVQIVKVFTERFQTIKRDKGMVDFTDLEHFCLQILSERTEEGELRPSPVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTPEGIDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGETVGEIDYDADAELKLGASYPEGEDVAAELLCIHQSEGEESAEGEDGVEVEKAQLEARL
MAGRIKAMVDSDYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVTVMMNVFRVID
NPMQDIPLAAVLRSPIVGLHDEELAMLRAHEKKGSFYEVMRSFLRGAPLEEEAELHGKLQWFYNLLQGWREFARQQSLSD
LIWKVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMT
IHKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALT
RAKEKLILIGTVKDKEKEMEKWLDAREHIDWLLPDYVRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPSEIYEYDASW
KVEVVDGNTLLAPEPVQEEKQELLEALREKRAVPLESERKEEVYARLTWEYAHEGATMHRAKQSVTEIKRNYQSEDGSDH
AFIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVNLQKPITPEVITEQIAEMVNKELLTFEQAEEITIDRIVDFFD
TDLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGQSKESVLVQGVIDCMIEEEDGIVLIDFKTDTIEGRFPGGYDQARPI
LEERYKVQLSLYAKALEKSLHHPVKEKYLYFFDGNHIVNVD

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=500412 IQ680_RS13370 WP_243521100.1 2595736..2599461(+) (addA) [Bacillus pseudomycoides strain N451-2]
ATGATAGAGAATTGGCCACAAAAGCCTGAAGGTAGTCAATGGACGGATGATCAATGGAAGGCAGTTGTCAGAGATGGCCG
TGATATTTTAGTCGCGGCGGCAGCTGGGTCGGGAAAGACAGCTGTACTTGTAGAAAGAATTATTCGCAAAATTATTAATG
CCGAGAATCCAGTAGATGTAGATCGTCTGCTTGTCGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAGAACAGAATT
GGCGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGATCACAGCACATTCGGAAGCAGCTTAGTTTATTAAATAAAGC
ATCGATTTCAACGATTCACTCGTTCTGTTTGCAAGTCATTCGAACATATTATTATATGCTCGACGTTGATCCGCGTTTTC
GCATTGCAAACCAGACAGAAAATGAATTATTAAAAGAAGAAGTATTAGATGACATATTAGAAGAGGAATATGGCACAGAG
GGAAATGATATTTTCTTTGAGCTCGTTGATCGCTATACGAGTGACCGAAGTGATGATGACTTGCAGCGCATGATTTTAGC
GCTTCATACAGAATCAAGAGCACATCCCGATCCAGAGAAATGGCTGGATAAGCTTGTTGAAGCATATGATGTGGAAGGAA
AATCAATTGAAGATTTAACGTATGCATCTTATTTATTAGAAGATGTGAAGTTTCAGCTGGAAACTGCTGAAAAGCATGTT
CAAAAAGCAATTGAACTTGCTATGCTTCCAGATGGACCAGCACCTCGCGCGGAGACATTACAAGCGGACATTATGTTACT
CGGAACGTTATCCAGTGCAGCGCAGCAGTCTTGGACAAGTGTATATGAAGCGATGCAAAATGTTTCATGGCAAACGTTAA
AGCGCATTAAGAAGAGTGACTACAATGAAGAGATTGTCAAACAAGTAGATTCACTTAGAAATAAAGCAAAAGATGAAGTG
AAGAAACTACAAGAAGAGTTATTTAGCCGAAAACCAGAAAGTTTTTTACGTGATTTTAAGGAGATGCACCCTGTACTAGA
AAAGCTTGTTCAAATTGTAAAAGTATTTACAGAGCGCTTCCAAACGATCAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTTTGCTTACAAATTTTAAGTGAACGTACAGAAGAGGGCGAGCTTCGTCCGTCGCCAGTTGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTACTTGTCGATGAATATCAAGATACAAACTTTGTCCAGGAGTCGATTATTAAGTTTGT
AACGAAAGACTCTGAGCAAGAAGGTAATCTGTTTATGGTTGGTGACGTAAAGCAGTCGATATATCGCTTCCGATTAGCAG
AACCTGGCTTGTTTTTAGGGAAATATAAACGATTTACGCCTGAAGGAATAGACGGCGGTATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCACGAAGTGTTAGCGGGGACGAACTTTATTTTTAAACAAATTATGGGCGAGACTGTCGGAGAAAT
CGATTATGATGCCGATGCGGAATTAAAGTTAGGTGCAAGTTATCCAGAAGGGGAAGATGTAGCAGCCGAGCTTTTATGTA
TTCATCAGTCTGAGGGGGAAGAATCGGCAGAGGGAGAAGACGGTGTAGAAGTAGAAAAAGCACAGCTGGAAGCGCGTTTA
ATGGCCGGGAGAATTAAAGCGATGGTGGATTCAGATTATGAAGTATACGATCGTAAAACAGATAGTATGCGCCCGGTGAA
ATATCGTGATTTTGTCATTTTACTACGCTCAATGCCGTGGGCTCCGCAAATTATGGAGGAGTTAAAATTACAAGGTATTC
CAGTTTACGCTGAGCTTGCGACGGGTTATTTTGAAGCAACAGAAGTGACAGTTATGATGAATGTATTTCGCGTAATTGAT
AATCCAATGCAAGATATTCCGCTTGCTGCTGTACTTCGTTCTCCGATTGTTGGATTACATGATGAGGAACTTGCGATGCT
TCGTGCTCATGAAAAGAAAGGTTCGTTTTATGAAGTGATGCGTTCCTTCTTACGAGGAGCACCGCTTGAGGAAGAGGCAG
AATTACACGGAAAACTGCAATGGTTTTATAACCTTCTGCAAGGGTGGCGCGAATTTGCACGTCAGCAGTCTCTTTCAGAT
TTAATTTGGAAAGTATATCGTGAGACAGGTTATTACGATTTCGTTGGTGGCCTCCCAGCAGGAAAGCAGCGCCAAGCGAA
CTTACGTGTATTATACGACCGGGCAAGGCAGTATGAAGCAACATCTTTCCGCGGTTTATTCCGTTTCCTTCGTTTTATTG
AACGTATTTTAGAGCGCGGGGACGATATGGGAACAGCGAGAGCGCTTGGCGAGCAAGAAGATGTTGTTCGAATTATGACG
ATTCATAAAAGTAAAGGATTAGAGTTCCCGGTAGTATTTGTAGCGGGACTTGGGCGCCGTTTTAATACACAAGATTTAAT
GAAACGTTTTTTACTGCACAAGGACTTCGGTTTTGGATCGCAGTTTATTGATCCTCGTAAACGAATTAAATATACGACGC
TATCACAATTAGCCATTAAACGTAAGATGAAGATGGAACTCATTGCCGAAGAGATGCGCGTATTATATGTTGCTTTAACG
CGTGCGAAAGAAAAATTGATTTTAATTGGTACAGTGAAAGATAAAGAGAAAGAAATGGAAAAGTGGCTTGATGCGCGTGA
ACATATAGATTGGTTATTACCAGATTACGTTCGTGCAGGGGCATCTTGTTATTTAGATTGGATTGCCCCATCACTATATA
GACATCGTGATAGTGAAATGCTTCTTGAACTTGGACAAGGCAATATACCAAGTGAAATTTATGAATACGATGCGAGCTGG
AAAGTGGAAGTCGTTGATGGTAACACTTTACTTGCTCCTGAGCCAGTGCAAGAAGAAAAGCAAGAATTGTTAGAAGCGCT
TCGTGAGAAAAGAGCGGTTCCGCTAGAAAGTGAACGAAAAGAAGAGGTGTATGCTCGTTTAACATGGGAATATGCGCATG
AAGGTGCAACAATGCACCGAGCGAAGCAATCTGTCACGGAGATTAAGCGAAATTATCAATCGGAAGATGGAAGTGATCAT
GCTTTTATTAAAAAGCTCCGTGCTCCTATAAAGACGCGTCCTCGTTTTATGGAGAAAAAGGGATTAACCTATGCTGAACG
GGGGACAGCCGTCCACGCCGTCATGCAGCATGTGAATCTACAAAAACCGATTACACCAGAAGTCATTACAGAACAAATTG
CGGAAATGGTAAATAAAGAATTATTAACATTTGAGCAAGCAGAAGAAATTACGATTGATCGTATTGTAGACTTTTTTGAT
ACGGATCTAGGTAAGCGTGTATTAGCTGCGAAAAGTGTAGAACGTGAAGTACCATTTACGATGATGCTTTCAGCGGAAGA
GGCATATCAAGATTGGCAAGGTCAGAGCAAAGAATCTGTACTTGTCCAAGGGGTTATTGATTGTATGATAGAAGAGGAAG
ATGGAATTGTATTAATTGATTTTAAAACAGATACAATTGAAGGGCGTTTTCCAGGCGGATACGATCAAGCAAGACCTATT
TTAGAAGAGCGTTATAAAGTGCAGCTTTCATTGTATGCAAAAGCGCTAGAGAAAAGCTTACATCATCCTGTAAAAGAAAA
ATATTTATACTTCTTTGATGGGAATCACATAGTAAATGTCGATTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1061-1229)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-891)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.606

100

0.539