Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IQ680_RS08165 Genome accession   NZ_CP064266
Coordinates   1548997..1550373 (+) Length   458 a.a.
NCBI ID   WP_243525426.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain N451-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1543997..1555373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ680_RS08145 ctsR 1544216..1544677 (+) 462 WP_243525422.1 transcriptional regulator CtsR -
  IQ680_RS08150 - 1544826..1545374 (+) 549 WP_243525423.1 UvrB/UvrC motif-containing protein -
  IQ680_RS08155 - 1545379..1546443 (+) 1065 WP_243525424.1 protein arginine kinase -
  IQ680_RS08160 clpC 1546466..1548901 (+) 2436 WP_243525425.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  IQ680_RS08165 radA 1548997..1550373 (+) 1377 WP_243525426.1 DNA repair protein RadA Machinery gene
  IQ680_RS08170 disA 1550377..1551450 (+) 1074 WP_000392160.1 DNA integrity scanning diadenylate cyclase DisA -
  IQ680_RS08175 - 1551611..1552729 (+) 1119 WP_243525427.1 PIN/TRAM domain-containing protein -
  IQ680_RS08180 ispD 1552746..1553429 (+) 684 WP_243525428.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IQ680_RS08185 ispF 1553536..1554012 (+) 477 WP_098338748.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49873.43 Da        Isoelectric Point: 6.8374

>NTDB_id=500405 IQ680_RS08165 WP_243525426.1 1548997..1550373(+) (radA) [Bacillus pseudomycoides strain N451-2]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEQVVSSRRINYANAIQAEVTKPRRLTEVETNSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIDEMNPSFVVIDSIQTIYLPEVTSAPGSVSQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=500405 IQ680_RS08165 WP_243525426.1 1548997..1550373(+) (radA) [Bacillus pseudomycoides strain N451-2]
ATGGCTAAAAAGAAAACGAAGTTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGGAAGTGTCCTGGATG
TGGACAATGGAACACTCTTGTTGAAGAAATGGAGCAGGTTGTGTCCTCGCGTCGTATCAACTATGCAAATGCGATTCAAG
CGGAAGTAACGAAACCAAGACGTCTTACAGAAGTAGAGACAAATTCAGAAGCGCGGATTGAAACAAAATTTCAAGAATTT
AATCGTGTGCTTGGTGGAGGAATTGTAGATGGTTCCCTCGTACTTATTGGAGGAGACCCTGGAATTGGGAAATCAACCCT
ATTACTACAAATATCATCGCAATTAGCAGATTCTTCGTATGATGTATTATATATATCTGGTGAAGAATCAGCAAAACAAA
TTAAACTTCGTGCAGATCGTCTACATGTAAATGGGAGCAATTTATTCGTTGTTTCGGAAACGGATTTACAGAGAATTGCG
GCACATATTGATGAAATGAATCCTTCCTTTGTTGTCATTGATTCCATTCAAACAATTTATTTACCAGAAGTAACTTCAGC
GCCAGGAAGTGTTTCGCAAGTACGTGAATGTACAGCAGAACTGATGAAACTTGCAAAAACGAAAGGGATTCCGATTTTTA
TCGTAGGGCATGTAACGAAAGAAGGTGCAATTGCAGGACCGCGTATGTTAGAACATATGGTGGATGCGGTTCTTTATTTT
GAGGGAGACCGTCATCATACATATCGTATTTTACGGGCTGTAAAAAATCGTTTTGGTTCTACAAATGAAATGGGCATATT
TGAAATGAAGGAATTAGGTCTTGCAGAAGTATTAAATCCTTCTGAGATTTTTCTAGAAGAGAGACCTGTTGGAGTTGCTG
GTTCTACAGTTGTTGCTTCTATGGAAGGAACAAGACCAGTTTTAGTAGAAATCCAAGCGTTAATTTCTCCTACTAGCTTT
GGAAATCCGCGAAGAATGGCTACAGGAATTGATCATAATCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAGCAGG
TTTATTATTACAAAATCAAGATGCTTATTTAAAGGTTGCTGGAGGATTAAAATTGGATGAACCAGCAATTGATTTAGCGG
TTGCTTTAAGTATTGCATCGAGTTTTCGAGATAAGTCCACTGCACCAACCGATGCGGTGATAGGAGAAGTTGGATTAACC
GGAGAAATAAGAAGGGTATCAAGAATTGAACAACGCGTACAAGAAGCAGCTAAGTTAGGATTTCAGCGTGCCATCATCCC
GCGAAAAAATTTAGGGGGATGGACAATTCCTGATGGGATTGAAGTAGTTGGCGTTTCTAATTTAGGGGAGGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(61-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631