Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   IRJ28_RS22095 Genome accession   NZ_CP064096
Coordinates   4211336..4212727 (+) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain N1142-3at     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4206336..4217727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS22075 yvyE 4207488..4208141 (-) 654 WP_003227979.1 YigZ family protein -
  IRJ28_RS22080 degS 4208358..4209515 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  IRJ28_RS22085 degU 4209598..4210287 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  IRJ28_RS22090 fakBA 4210385..4211230 (+) 846 WP_003244125.1 DegV family protein -
  IRJ28_RS22095 comFA 4211336..4212727 (+) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  IRJ28_RS22100 comFB 4212787..4213083 (+) 297 WP_003227989.1 late competence protein ComFB -
  IRJ28_RS22105 comFC 4213047..4213769 (+) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  IRJ28_RS22110 yvyF 4213843..4214262 (+) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  IRJ28_RS22115 flgM 4214343..4214609 (+) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  IRJ28_RS22120 flgN 4214625..4215107 (+) 483 WP_003227999.1 flagellar protein FlgN -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=499521 IRJ28_RS22095 WP_003243962.1 4211336..4212727(+) (comFA) [Bacillus subtilis strain N1142-3at]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=499521 IRJ28_RS22095 WP_003243962.1 4211336..4212727(+) (comFA) [Bacillus subtilis strain N1142-3at]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1