Detailed information NTG0499515

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: IRJ28_RS21035
• Genome accession: NZ_CP064096
• Coordinates: 4007720..4008865 (-)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain N1142-3at
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 4002720..4013865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ28_RS21010	pbpG	4002973..4005048 (-)	2076	WP_003227540.1	transglycosylase domain-containing protein	-
IRJ28_RS21015	speE	4005250..4006080 (+)	831	WP_003227543.1	spermidine synthase	-
IRJ28_RS21020	speB	4006141..4007013 (+)	873	WP_003227545.1	agmatinase	-
IRJ28_RS21025	ywhH	4007045..4007518 (-)	474	WP_003227547.1	YbaK/EbsC family protein	-
IRJ28_RS21030	phrF	4007617..4007736 (-)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
IRJ28_RS21035	rapF	4007720..4008865 (-)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
IRJ28_RS22165	-	4008868..4009098 (+)	231	WP_009968328.1	hypothetical protein	-
IRJ28_RS21040	ywhK	4009095..4010450 (+)	1356	WP_003227552.1	YncE family protein	-
IRJ28_RS21045	ywhL	4010489..4011865 (+)	1377	WP_003244415.1	YncE family protein	-
IRJ28_RS21050	albG	4011871..4012572 (-)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
IRJ28_RS21055	albF	4012569..4013849 (-)	1281	WP_003242974.1	pitrilysin family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=499515 IRJ28_RS21035 WP_003227549.1 4007720..4008865(-) (rapF) [Bacillus subtilis strain N1142-3at]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=499515 IRJ28_RS21035 WP_003227549.1 4007720..4008865(-) (rapF) [Bacillus subtilis strain N1142-3at]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572