Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IRJ24_RS14180 Genome accession   NZ_CP064093
Coordinates   2709767..2710837 (+) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain N1282-4at     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704767..2715837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ24_RS14150 yqgY 2704961..2705206 (-) 246 WP_003226300.1 DUF2626 domain-containing protein -
  IRJ24_RS14155 mgsR 2705438..2705818 (+) 381 WP_003230147.1 transcriptional regulator MgsR -
  IRJ24_RS14165 rsbRD 2706042..2706878 (+) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  IRJ24_RS14170 yqhB 2706931..2708259 (-) 1329 WP_009967730.1 hemolysin family protein -
  IRJ24_RS14175 corA 2708402..2709355 (+) 954 WP_004399136.1 magnesium transporter CorA -
  IRJ24_RS14180 comGA 2709767..2710837 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  IRJ24_RS14185 comGB 2710824..2711861 (+) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  IRJ24_RS14190 comGC 2711875..2712171 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  IRJ24_RS14195 comGD 2712161..2712592 (+) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  IRJ24_RS14200 comGE 2712576..2712923 (+) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  IRJ24_RS14205 comGF 2712949..2713332 (+) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  IRJ24_RS14210 comGG 2713333..2713707 (+) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  IRJ24_RS14215 spoIIT 2713778..2713957 (+) 180 WP_003230176.1 YqzE family protein -
  IRJ24_RS14220 yqzG 2713999..2714325 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  IRJ24_RS14225 tapA 2714597..2715358 (+) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=499234 IRJ24_RS14180 WP_004399124.1 2709767..2710837(+) (comGA) [Bacillus subtilis strain N1282-4at]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=499234 IRJ24_RS14180 WP_004399124.1 2709767..2710837(+) (comGA) [Bacillus subtilis strain N1282-4at]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1