Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IRJ24_RS14185 Genome accession   NZ_CP064093
Coordinates   2710824..2711861 (+) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis strain N1282-4at     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705824..2716861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ24_RS14165 rsbRD 2706042..2706878 (+) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  IRJ24_RS14170 yqhB 2706931..2708259 (-) 1329 WP_009967730.1 hemolysin family protein -
  IRJ24_RS14175 corA 2708402..2709355 (+) 954 WP_004399136.1 magnesium transporter CorA -
  IRJ24_RS14180 comGA 2709767..2710837 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  IRJ24_RS14185 comGB 2710824..2711861 (+) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  IRJ24_RS14190 comGC 2711875..2712171 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  IRJ24_RS14195 comGD 2712161..2712592 (+) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  IRJ24_RS14200 comGE 2712576..2712923 (+) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  IRJ24_RS14205 comGF 2712949..2713332 (+) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  IRJ24_RS14210 comGG 2713333..2713707 (+) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  IRJ24_RS14215 spoIIT 2713778..2713957 (+) 180 WP_003230176.1 YqzE family protein -
  IRJ24_RS14220 yqzG 2713999..2714325 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  IRJ24_RS14225 tapA 2714597..2715358 (+) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  IRJ24_RS14230 sipW 2715342..2715914 (+) 573 WP_003246088.1 signal peptidase I -
  IRJ24_RS14235 tasA 2715978..2716763 (+) 786 WP_004398632.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=499235 IRJ24_RS14185 WP_009967727.1 2710824..2711861(+) (comGB) [Bacillus subtilis strain N1282-4at]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=499235 IRJ24_RS14185 WP_009967727.1 2710824..2711861(+) (comGB) [Bacillus subtilis strain N1282-4at]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936