Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JFX80_RS12240 Genome accession   NZ_CP075632
Coordinates   2461497..2461937 (+) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 2018-166     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2456497..2466937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFX80_RS12220 (JFX80_12230) aroP 2457495..2458868 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  JFX80_RS12225 (JFX80_12235) ampE 2458911..2459765 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  JFX80_RS12230 (JFX80_12240) ampD 2459762..2460313 (-) 552 WP_000923735.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JFX80_RS12235 (JFX80_12245) nadC 2460401..2461294 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JFX80_RS12240 (JFX80_12250) pilA 2461497..2461937 (+) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  JFX80_RS12245 (JFX80_12255) pilB 2461947..2463332 (+) 1386 WP_062863566.1 type II secretion system protein GspE Machinery gene
  JFX80_RS12250 (JFX80_12260) hofC 2463322..2464242 (+) 921 Protein_2421 protein transport protein HofC -
  JFX80_RS12255 (JFX80_12265) - 2464371..2465108 (-) 738 WP_000528252.1 hypothetical protein -
  JFX80_RS12260 (JFX80_12270) - 2465062..2465262 (-) 201 WP_001310452.1 Com family DNA-binding transcriptional regulator -
  JFX80_RS12265 (JFX80_12275) - 2465377..2465840 (+) 464 Protein_2424 hypothetical protein -
  JFX80_RS12270 (JFX80_12280) - 2465879..2466124 (-) 246 WP_001114107.1 DUF826 domain-containing protein -
  JFX80_RS12275 (JFX80_12285) - 2466160..2466342 (-) 183 WP_000144787.1 DUF1378 family protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=499174 JFX80_RS12240 WP_000360902.1 2461497..2461937(+) (pilA) [Escherichia coli strain 2018-166]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=499174 JFX80_RS12240 WP_000360902.1 2461497..2461937(+) (pilA) [Escherichia coli strain 2018-166]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377