Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ISX45_RS10360 Genome accession   NZ_CP064060
Coordinates   2071360..2072427 (-) Length   355 a.a.
NCBI ID   WP_181554393.1    Uniprot ID   A0A7V9Z3P1
Organism   Thermaerobacillus caldiproteolyticus strain U458     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2066360..2077427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX45_RS10325 (ISX45_10325) - 2066502..2067434 (+) 933 WP_194518919.1 hypothetical protein -
  ISX45_RS10330 (ISX45_10330) - 2067470..2068582 (-) 1113 WP_194518920.1 DNA cytosine methyltransferase -
  ISX45_RS10335 (ISX45_10335) - 2068659..2068967 (-) 309 WP_194518921.1 helix-turn-helix domain-containing protein -
  ISX45_RS10340 (ISX45_10340) - 2069067..2069399 (-) 333 WP_194518922.1 sigma factor-like helix-turn-helix DNA-binding protein -
  ISX45_RS10345 (ISX45_10345) - 2069368..2069697 (-) 330 WP_194518923.1 sigma-70 family RNA polymerase sigma factor -
  ISX45_RS10350 (ISX45_10350) comGC 2070182..2070319 (-) 138 Protein_2105 competence type IV pilus major pilin ComGC -
  ISX45_RS10355 (ISX45_10355) comGB 2070336..2071367 (-) 1032 WP_181554392.1 competence type IV pilus assembly protein ComGB -
  ISX45_RS10360 (ISX45_10360) comGA 2071360..2072427 (-) 1068 WP_181554393.1 competence type IV pilus ATPase ComGA Machinery gene
  ISX45_RS10365 (ISX45_10365) - 2072632..2073330 (+) 699 WP_194518924.1 helix-turn-helix transcriptional regulator -
  ISX45_RS10370 (ISX45_10370) - 2073411..2073653 (+) 243 WP_194518925.1 DUF2626 domain-containing protein -
  ISX45_RS10375 (ISX45_10375) - 2073799..2074926 (-) 1128 WP_194518926.1 class I SAM-dependent methyltransferase -
  ISX45_RS10380 (ISX45_10380) - 2075617..2076249 (-) 633 WP_194518927.1 MBL fold metallo-hydrolase -
  ISX45_RS10385 (ISX45_10385) - 2076347..2076778 (+) 432 WP_181554398.1 hypothetical protein -
  ISX45_RS10390 (ISX45_10390) - 2076846..2077022 (+) 177 WP_181554399.1 DUF2759 domain-containing protein -
  ISX45_RS10395 (ISX45_10395) - 2077055..2077366 (-) 312 WP_181554400.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40132.88 Da        Isoelectric Point: 9.6181

>NTDB_id=498888 ISX45_RS10360 WP_181554393.1 2071360..2072427(-) (comGA) [Thermaerobacillus caldiproteolyticus strain U458]
MNEIEQLADRLIKEASVLKASDIHVVPRREDAVIQFRVDGVLSRKEILTKEKCERLVAHFKFLAGMDIGERRRPQSGAME
IKQNDMIVSLRLSTLPTVYDESLVIRLLPHDSFLPLSQLSLFPNTTKKLLSLLHHSHGLMIFTGPTGSGKTTTLYTLLRV
CQDKWNRNVITLEDPIEKKTDHVLQVQVNEKAGITYATGLKAILRHDPDVIMVGEIRDAETARITVRAALTGHLVLSTLH
TNNALGAIYRLREFGIPWQDIEQTLLAVAAQRLVDIRCPLCQENCSPFCRNKRKVRRAAIYELLYGKALMKAIQAARGEK
VTYRYETLDDVIKKGIALGFLSPHLLMREGIKENV

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=498888 ISX45_RS10360 WP_181554393.1 2071360..2072427(-) (comGA) [Thermaerobacillus caldiproteolyticus strain U458]
ATGAACGAAATTGAACAGCTGGCTGACCGTTTAATCAAAGAAGCTAGCGTGTTGAAAGCATCTGATATTCATGTTGTGCC
GCGACGGGAAGATGCTGTCATTCAATTCCGTGTGGATGGAGTTTTGTCCAGAAAGGAAATCCTCACAAAAGAAAAATGTG
AGCGATTGGTTGCCCATTTTAAATTTTTAGCTGGGATGGACATTGGTGAGCGGCGCCGGCCGCAAAGTGGTGCAATGGAG
ATCAAGCAAAATGACATGATAGTTAGCTTGCGGTTATCAACGTTGCCGACAGTTTACGATGAAAGTCTCGTCATTCGTCT
TCTCCCTCATGACTCTTTTCTTCCTTTATCACAACTCTCTCTGTTTCCAAACACAACAAAAAAATTGTTATCCCTTCTCC
ATCATTCCCATGGATTAATGATTTTTACAGGGCCGACTGGTTCAGGAAAAACGACTACGTTATATACATTGCTGCGCGTA
TGTCAAGACAAGTGGAATCGCAATGTCATTACGCTTGAGGATCCTATTGAAAAGAAAACCGACCACGTTTTGCAAGTACA
AGTCAACGAAAAAGCAGGAATAACGTATGCGACTGGCTTAAAAGCGATTTTACGTCATGATCCCGACGTAATTATGGTCG
GGGAAATTCGCGACGCAGAAACAGCGAGAATTACGGTTCGCGCTGCCTTAACGGGTCATCTTGTATTATCTACCTTGCAT
ACGAACAATGCACTTGGAGCGATTTATCGACTACGCGAATTCGGTATACCGTGGCAAGACATTGAGCAGACATTGCTTGC
CGTTGCAGCGCAGCGTCTTGTAGACATAAGGTGCCCGCTCTGCCAAGAAAACTGCTCCCCATTTTGTCGAAACAAAAGAA
AAGTGCGGCGCGCGGCCATTTATGAGCTGCTGTATGGCAAGGCATTAATGAAAGCCATTCAAGCGGCACGAGGGGAGAAA
GTGACATACCGTTATGAAACGTTAGACGATGTAATAAAAAAAGGGATCGCGCTAGGATTTTTATCACCACATCTTCTTAT
GCGTGAGGGGATTAAAGAAAATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V9Z3P1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

60.058

96.62

0.58
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.922

97.746

0.4
  pilF Thermus thermophilus HB27

38.953

96.901

0.377