Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H9J98_RS01705 Genome accession   NZ_CP064041
Coordinates   359085..359678 (+) Length   197 a.a.
NCBI ID   WP_005496571.1    Uniprot ID   A0A0F2HLP7
Organism   Vibrio parahaemolyticus strain XMO116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354085..364678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9J98_RS01690 (H9J98_01685) - 354801..357371 (-) 2571 WP_100088106.1 PBP1A family penicillin-binding protein -
  H9J98_RS01695 (H9J98_01690) pilM 357514..358530 (+) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  H9J98_RS01700 (H9J98_01695) pilN 358514..359092 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  H9J98_RS01705 (H9J98_01700) pilO 359085..359678 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9J98_RS01710 (H9J98_01705) pilP 359668..360183 (+) 516 WP_025794802.1 pilus assembly protein PilP Machinery gene
  H9J98_RS01715 (H9J98_01710) pilQ 360215..361963 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9J98_RS01720 (H9J98_01715) aroK 362152..362670 (+) 519 WP_005381319.1 shikimate kinase AroK -
  H9J98_RS01725 (H9J98_01720) aroB 362702..363802 (+) 1101 WP_023622897.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23019.57 Da        Isoelectric Point: 4.9419

>NTDB_id=498632 H9J98_RS01705 WP_005496571.1 359085..359678(+) (pilO) [Vibrio parahaemolyticus strain XMO116]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDGK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=498632 H9J98_RS01705 WP_005496571.1 359085..359678(+) (pilO) [Vibrio parahaemolyticus strain XMO116]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTCGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCTAACAAGGTAGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTTACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGCATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGCATCATC
ACCTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGGGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2HLP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711