Detailed information NTG0498554

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H9K48_RS03005
• Genome accession: NZ_CP064033
• Coordinates: 614331..615200 (-)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus strain DLM1805
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 609331..620200

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9K48_RS02970 (H9K48_02965)	rpsP	610379..610627 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
H9K48_RS02975 (H9K48_02970)	rimM	610656..611204 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
H9K48_RS02980 (H9K48_02975)	trmD	611232..611975 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
H9K48_RS02985 (H9K48_02980)	rplS	612017..612370 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
H9K48_RS02990 (H9K48_02985)	yacG	612685..612879 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
H9K48_RS02995 (H9K48_02990)	zapD	612948..613688 (-)	741	WP_015297254.1	cell division protein ZapD	-
H9K48_RS03000 (H9K48_02995)	coaE	613716..614330 (-)	615	WP_005480887.1	dephospho-CoA kinase	-
H9K48_RS03005 (H9K48_03000)	pilD	614331..615200 (-)	870	WP_005493974.1	A24 family peptidase	Machinery gene
H9K48_RS03010 (H9K48_03005)	pilC	615265..616488 (-)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
H9K48_RS03015 (H9K48_03010)	pilB	616513..618198 (-)	1686	WP_025792845.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H9K48_RS03020 (H9K48_03015)	pilA	618204..618662 (-)	459	WP_025518565.1	pilin	Machinery gene
H9K48_RS03025 (H9K48_03020)	nadC	618925..619812 (-)	888	WP_015297249.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=498554 H9K48_RS03005 WP_005493974.1 614331..615200(-) (pilD) [Vibrio parahaemolyticus strain DLM1805]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=498554 H9K48_RS03005 WP_005493974.1 614331..615200(-) (pilD) [Vibrio parahaemolyticus strain DLM1805]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTGGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTAGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTAAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCTGTAATCAGCTGGCTACTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGATATCC
GCTGATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCCGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45